采用超高效液相色谱法测定动物饲料中的对位红、苏丹染料、角黄素和虾青素。

Determination of para red, Sudan dyes, canthaxanthin, and astaxanthin in animal feeds using UPLC.

作者信息

Hou Xiaolin, Li Yonggang, Wu Guojuan, Wang Lei, Hong Miao, Wu Yongnin

机构信息

Department of Animal Science, Beijing University of Agriculture, Beijing, PR China, 102206.

出版信息

J Chromatogr Sci. 2010 Jan;48(1):22-5. doi: 10.1093/chromsci/48.1.22.

DOI:10.1093/chromsci/48.1.22

PMID:20056031

Abstract

A simple high-performance liquid chromatography method was developed for quantitative determination of para red, Sudan I, Sudan II, Sudan III, Sudan IV, canthaxanthin, and astaxanthin in feedstuff. The sample was extracted using acetonitrile and cleaned up on a C(18) SPE column. The residues were analyzed using ultra-performance liquid chromatography coupled to a diode array detector at 500 nm. The mobile phase was acetonitrile-formic acid-water with a gradient elution condition. The external standard curves were calibrated. The mean recoveries of the seven colorants were 62.7-91.0% with relative standard deviation 2.6-10.4% (intra-day) and 4.0-13.2% (inter-day). The detection limits were in the range of 0.006-0.02 mg/kg.

摘要

建立了一种简单的高效液相色谱法，用于定量测定饲料中的对位红、苏丹红I、苏丹红II、苏丹红III、苏丹红IV、角黄素和虾青素。样品用乙腈提取，并在C(18)固相萃取柱上净化。残留物采用超高效液相色谱-二极管阵列检测器在500nm波长下进行分析。流动相为乙腈-甲酸-水，采用梯度洗脱条件。绘制了外标曲线。七种色素的平均回收率为62.7%-91.0%，日内相对标准偏差为2.6%-10.4%，日间相对标准偏差为4.0%-13.2%。检测限在0.006-0.02mg/kg范围内。