Division of Material Engineering, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa 920-1192, Japan.
Ultrason Sonochem. 2010 Apr;17(4):738-43. doi: 10.1016/j.ultsonch.2009.12.001. Epub 2009 Dec 5.
A non-woven titanium dioxide (TiO(2)) fabric was applied to disinfection by ultrasound (US) irradiation, and the disinfection efficiency and lipid peroxidation of Escherichia coli (E. coli) cell membrane were evaluated to investigate the killing process. The addition of non-woven TiO(2) fabric enhanced hydroxyl (OH) radical generation and disinfection efficiency. Judging from the disinfection experiments using glutathione or t-butanol as a radical scavenger, the OH radical played a major role in cell killing in sonodynamic disinfection with non-woven TiO(2) fabric. Moreover, to understand the detailed killing process, damage to cell membrane was also evaluated using a diphenyl-1-pyrenylphosphine (DPPP) fluorescent probe, which detects the membrane's lipid peroxidation. The addition of non-woven TiO(2) fabric aggravated this peroxidation. This aggravation was caused by the OH radical according to an assay using a radical scavenger. From these results, it was concluded that non-woven TiO(2) fabric as a sonocatalyst promoted peroxidation of the polyunsaturated phospholipid component of the lipid membrane initially and induced a major disorder in the E. coli cell membrane under US irradiation.
一种无纺二氧化钛（TiO(2)）织物被应用于超声（US）照射下的消毒，评估了其对大肠杆菌（E. coli）细胞膜的消毒效率和脂质过氧化作用，以研究其杀菌过程。添加无纺 TiO(2)织物增强了羟基（OH）自由基的生成和消毒效率。从使用谷胱甘肽或叔丁醇作为自由基清除剂的消毒实验判断，OH 自由基在使用无纺 TiO(2)织物的声动力学消毒中对细胞杀伤起主要作用。此外，为了了解详细的杀菌过程，还使用二苯基-1-芘基膦（DPPP）荧光探针评估细胞膜的损伤，该探针检测细胞膜的脂质过氧化作用。添加无纺 TiO(2)织物加剧了这种过氧化作用。根据使用自由基清除剂的测定，这种加剧是由 OH 自由基引起的。根据这些结果，可以得出结论，作为声催化剂的无纺 TiO(2)织物最初促进了多不饱和磷脂成分的脂质膜的过氧化，并在 US 照射下诱导大肠杆菌细胞膜发生主要紊乱。
