Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research), 3 Research Link, Singapore.
Nanotechnology. 2010 Feb 10;21(6):065101. doi: 10.1088/0957-4484/21/6/065101. Epub 2010 Jan 8.
Physically loading of paclitaxel (PTX) onto carbon nanotubes (CNTs) is achieved through immersion of poly(ethylene glycol) (PEG)-graft-single walled CNTs (PEG-g-SWNTs) or PEG-graft-multi-walled CNTs (PEG-g-MWNTs) in a saturated solution of PTX in methanol. After loading once the loading capacity (LD%) is 26% (w/w) and 36% (w/w) for PEG-g-SWNTs or PEG-g-MWNTs, respectively. With these PTX contents, PTX loaded PEG-g-SWNTs and PTX loaded PEG-g-MWNTs still have good dispersity in aqueous solution and individual CNTs can be observed in TEM images. PTX can be released from PEG-g-CNTs several times faster than from free PTX but still in a sustained profile with less than 40% of PTX being released in 40 days at pH 7 or 5. In vitro cytotoxicity of samples is evaluated in HeLa cells and MCF-7 cells. PEG-g-SWNTs and PEG-g-MWNTs show low cytotoxicity in both cells with insignificant effects on the cell proliferation rates. However, both PTX loaded PEG-g-SWNTs and PTX loaded PEG-g-MWNTs show high efficacy to kill HeLa cells and MCF-7 cells, as reflected by IC(50) lower than free PTX. Therefore, PTX loaded PEG-g-CNTs are promising for cancer therapeutics.
carbon nanotubes, poly(ethylene glycol), drug delivery, cancer therapy, nanomedicine.
通过将聚乙二醇（PEG）-接枝单壁碳纳米管（PEG-g-SWNTs）或 PEG-接枝多壁碳纳米管（PEG-g-MWNTs）浸入紫杉醇（PTX）在甲醇中的饱和溶液中，实现了将紫杉醇物理加载到碳纳米管（CNTs）上。负载一次后，PEG-g-SWNTs 或 PEG-g-MWNTs 的负载量（LD%）分别为 26%（w/w）和 36%（w/w）。在这些 PTX 含量下，负载 PTX 的 PEG-g-SWNTs 和负载 PTX 的 PEG-g-MWNTs 在水溶液中仍具有良好的分散性，并且可以在 TEM 图像中观察到单个 CNTs。与游离 PTX 相比，PTX 从 PEG-g-CNTs 中的释放速度快几倍，但仍呈持续释放模式，在 pH7 或 5 下 40 天内释放少于 40%的 PTX。在 HeLa 细胞和 MCF-7 细胞中评估了样品的体外细胞毒性。PEG-g-SWNTs 和 PEG-g-MWNTs 在两种细胞中均显示出低细胞毒性，对细胞增殖率没有明显影响。然而，负载 PTX 的 PEG-g-SWNTs 和负载 PTX 的 PEG-g-MWNTs 对 HeLa 细胞和 MCF-7 细胞均具有高效的杀伤作用，IC（50）低于游离 PTX。因此，负载 PTX 的 PEG-g-CNTs 有望用于癌症治疗。
碳纳米管、聚乙二醇、药物输送、癌症治疗、纳米医学。
