• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

DAP5的MIF4G结构域的结晶及初步X射线衍射分析

Crystallization and preliminary X-ray diffraction analysis of the MIF4G domain of DAP5.

作者信息

Frank Filipp, Virgili Geneviève, Sonenberg Nahum, Nagar Bhushan

机构信息

Department of Biochemistry, McGill University, Montréal, Québec, Canada.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jan 1;66(Pt 1):15-9. doi: 10.1107/S1744309109044315. Epub 2009 Dec 25.

DOI:10.1107/S1744309109044315
PMID:20057060
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2805526/
Abstract

Death-associated protein 5 (DAP5) is a member of the eIF4G family of scaffolding proteins that mediate cap-independent translation initiation by recruiting the translational machinery to internal ribosomal entry sites (IRESs) on mRNA. The MIF4G domain of DAP5 directly interacts with the eukaryotic initiation factors eIF4A and eIF3 and enhances the translation of several viral and cellular IRESs. Here, the crystallization and preliminary X-ray diffraction analysis of the MIF4G domain of DAP5 is presented.

摘要

死亡相关蛋白5（DAP5）是支架蛋白eIF4G家族的成员，通过将翻译机制募集到mRNA上的内部核糖体进入位点（IRES）来介导不依赖帽的翻译起始。DAP5的MIF4G结构域直接与真核起始因子eIF4A和eIF3相互作用，并增强几种病毒和细胞IRES的翻译。本文介绍了DAP5的MIF4G结构域的结晶及初步X射线衍射分析。

相似文献

1
Crystallization and preliminary X-ray diffraction analysis of the MIF4G domain of DAP5.DAP5的MIF4G结构域的结晶及初步X射线衍射分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jan 1;66(Pt 1):15-9. doi: 10.1107/S1744309109044315. Epub 2009 Dec 25.
2
Structural analysis of the DAP5 MIF4G domain and its interaction with eIF4A.DAP5 MIF4G 结构域分析及其与 eIF4A 的相互作用。
Structure. 2013 Apr 2;21(4):517-27. doi: 10.1016/j.str.2013.01.015. Epub 2013 Mar 7.
3
Crystal structure of the MIF4G domain of the Trypanosoma cruzi translation initiation factor EIF4G5.克氏锥虫翻译起始因子EIF4G5的MIF4G结构域的晶体结构
Acta Crystallogr F Struct Biol Commun. 2019 Dec 1;75(Pt 12):738-743. doi: 10.1107/S2053230X19015061. Epub 2019 Nov 20.
4
Modeling the structure and DAP5-binding site of the FGF-9 5'-UTR RNA utilized in cap-independent translation.模拟在无帽依赖翻译中使用的 FGF-9 5'-UTR RNA 的结构和 DAP5 结合位点。
RNA. 2024 Aug 16;30(9):1184-1198. doi: 10.1261/rna.080013.124.
5
A novel form of DAP5 protein accumulates in apoptotic cells as a result of caspase cleavage and internal ribosome entry site-mediated translation.一种新型的DAP5蛋白由于半胱天冬酶切割和内部核糖体进入位点介导的翻译而在凋亡细胞中积累。
Mol Cell Biol. 2000 Jan;20(2):496-506. doi: 10.1128/MCB.20.2.496-506.2000.
6
The crystal structure of the C-terminal DAP5/p97 domain sheds light on the molecular basis for its processing by caspase cleavage.C 端 DAP5/p97 结构域的晶体结构揭示了其经半胱天冬酶切割加工的分子基础。
J Mol Biol. 2008 Nov 14;383(3):539-48. doi: 10.1016/j.jmb.2008.08.013. Epub 2008 Aug 12.
7
The caspase-cleaved DAP5 protein supports internal ribosome entry site-mediated translation of death proteins.半胱天冬酶切割的DAP5蛋白支持内部核糖体进入位点介导的死亡蛋白翻译。
Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5400-5. doi: 10.1073/pnas.082102499. Epub 2002 Apr 9.
8
Translation initiation factor eIF4G-1 binds to eIF3 through the eIF3e subunit.翻译起始因子eIF4G-1通过eIF3e亚基与eIF3结合。
J Biol Chem. 2006 Aug 11;281(32):22917-32. doi: 10.1074/jbc.M605418200. Epub 2006 Jun 9.
9
Direct functional interaction of initiation factor eIF4G with type 1 internal ribosomal entry sites.起始因子eIF4G与1型内部核糖体进入位点的直接功能相互作用。
Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9197-202. doi: 10.1073/pnas.0900153106. Epub 2009 May 22.
10
5'-UTR recruitment of the translation initiation factor eIF4GI or DAP5 drives cap-independent translation of a subset of human mRNAs.5'-UTR 招募翻译起始因子 eIF4GI 或 DAP5 驱动一组人类 mRNA 的 cap 非依赖性翻译。
J Biol Chem. 2020 Aug 14;295(33):11693-11706. doi: 10.1074/jbc.RA120.013678. Epub 2020 Jun 22.

引用本文的文献

1
A newly identified Leishmania IF4E-interacting protein, Leish4E-IP2, modulates the activity of cap-binding protein paralogs.一种新鉴定的与利什曼原虫 IF4E 相互作用的蛋白，Leish4E-IP2，调节帽结合蛋白同工型的活性。
Nucleic Acids Res. 2020 May 7;48(8):4405-4417. doi: 10.1093/nar/gkaa173.
2
Cleavage of DAP5 by coxsackievirus B3 2A protease facilitates viral replication and enhances apoptosis by altering translation of IRES-containing genes.柯萨奇病毒B3 2A蛋白酶对DAP5的切割通过改变含内部核糖体进入位点（IRES）基因的翻译促进病毒复制并增强细胞凋亡。
Cell Death Differ. 2016 May;23(5):828-40. doi: 10.1038/cdd.2015.145. Epub 2015 Nov 20.
3
Structural analysis of the DAP5 MIF4G domain and its interaction with eIF4A.DAP5 MIF4G 结构域分析及其与 eIF4A 的相互作用。
Structure. 2013 Apr 2;21(4):517-27. doi: 10.1016/j.str.2013.01.015. Epub 2013 Mar 7.

本文引用的文献

1
Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
The crystal structure of the C-terminal DAP5/p97 domain sheds light on the molecular basis for its processing by caspase cleavage.C 端 DAP5/p97 结构域的晶体结构揭示了其经半胱天冬酶切割加工的分子基础。
J Mol Biol. 2008 Nov 14;383(3):539-48. doi: 10.1016/j.jmb.2008.08.013. Epub 2008 Aug 12.
3
Crystal structure of the yeast eIF4A-eIF4G complex: an RNA-helicase controlled by protein-protein interactions.酵母eIF4A-eIF4G复合物的晶体结构：一种受蛋白质-蛋白质相互作用控制的RNA解旋酶。
Proc Natl Acad Sci U S A. 2008 Jul 15;105(28):9564-9. doi: 10.1073/pnas.0800418105. Epub 2008 Jul 7.
4
DAP5 promotes cap-independent translation of Bcl-2 and CDK1 to facilitate cell survival during mitosis.DAP5促进Bcl-2和CDK1的非帽依赖性翻译，以促进有丝分裂期间的细胞存活。
Mol Cell. 2008 May 23;30(4):447-59. doi: 10.1016/j.molcel.2008.03.018. Epub 2008 May 1.
5
The eIF4G homolog DAP5/p97 supports the translation of select mRNAs during endoplasmic reticulum stress.真核起始因子4G（eIF4G）同源物DAP5/p97在内质网应激期间支持特定mRNA的翻译。
Nucleic Acids Res. 2008 Jan;36(1):168-78. doi: 10.1093/nar/gkm1007. Epub 2007 Nov 14.
6
Regulation of poly(A)-binding protein through PABP-interacting proteins.通过多聚腺苷酸结合蛋白（PABP）相互作用蛋白对多聚腺苷酸结合蛋白的调控。
Cold Spring Harb Symp Quant Biol. 2006;71:537-43. doi: 10.1101/sqb.2006.71.061.
7
Eukaryotic translation initiation factor 4GI and p97 promote cellular internal ribosome entry sequence-driven translation.真核生物翻译起始因子4GI和p97促进细胞内部核糖体进入位点驱动的翻译。
Proc Natl Acad Sci U S A. 2005 Sep 20;102(38):13421-6. doi: 10.1073/pnas.0506536102. Epub 2005 Sep 7.
8
Translational control in stress and apoptosis.应激与细胞凋亡中的翻译调控
Nat Rev Mol Cell Biol. 2005 Apr;6(4):318-27. doi: 10.1038/nrm1618.
9
Refinement of macromolecular structures by the maximum-likelihood method.用最大似然法优化大分子结构。
Acta Crystallogr D Biol Crystallogr. 1997 May 1;53(Pt 3):240-55. doi: 10.1107/S0907444996012255.
10
Improvement of macromolecular electron-density maps by the simultaneous application of real and reciprocal space constraints.通过同时应用实空间和倒易空间约束来改进大分子电子密度图。
Acta Crystallogr D Biol Crystallogr. 1993 Jan 1;49(Pt 1):148-57. doi: 10.1107/S0907444992007698.