酵母核糖核酸酶MRP的P3 RNA结构域与核糖核酸酶P/MRP蛋白组分Pop6和Pop7形成复合物的结晶及初步X射线衍射分析。
Crystallization and preliminary X-ray diffraction analysis of the P3 RNA domain of yeast ribonuclease MRP in a complex with RNase P/MRP protein components Pop6 and Pop7.
作者信息
Perederina Anna, Esakova Olga, Quan Chao, Khanova Elena, Krasilnikov Andrey S
机构信息
Department of Biochemistry and Molecular Biology, Pennsylvania State University, USA.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jan 1;66(Pt 1):76-80. doi: 10.1107/S1744309109049707. Epub 2009 Dec 25.
Abstract
Eukaryotic ribonucleases P and MRP are closely related RNA-based enzymes which contain a catalytic RNA component and several protein subunits. The roles of the protein subunits in the structure and function of eukaryotic ribonucleases P and MRP are not clear. Crystals of a complex that included a circularly permuted 46-nucleotide-long P3 domain of the RNA component of Saccharomyces cerevisiae ribonuclease MRP and selenomethionine derivatives of the shared ribonuclease P/MRP protein components Pop6 (18.2 kDa) and Pop7 (15.8 kDa) were obtained using the sitting-drop vapour-diffusion method. The crystals belonged to space group P4(2)22 (unit-cell parameters a = b = 127.2, c = 76.8 A, alpha = beta = gamma = 90 degrees ) and diffracted to 3.25 A resolution.
摘要
真核核糖核酸酶P和MRP是密切相关的基于RNA的酶，它们含有一个催化RNA组分和几个蛋白质亚基。蛋白质亚基在真核核糖核酸酶P和MRP的结构和功能中的作用尚不清楚。利用坐滴气相扩散法获得了一种复合物的晶体，该复合物包括酿酒酵母核糖核酸酶MRP的RNA组分的一个环形排列的46个核苷酸长的P3结构域以及共享的核糖核酸酶P/MRP蛋白质组分Pop6（18.2 kDa）和Pop7（15.8 kDa）的硒代蛋氨酸衍生物。这些晶体属于空间群P4(2)22（晶胞参数a = b = 127.2，c = 76.8 Å，α = β = γ = 90°），衍射分辨率为3.25 Å。
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