体内标记后，各种低电离辐射对诺维科夫肝癌腹水细胞总细胞RNA和核糖体前体核RNA的影响。

The effects of various kinds of sparsely-ionizing radiation on total cell RNA and preribosomal nuclear RNA of Novikoff hepatoma ascites cells after in vivo labelling.

作者信息

Schmitt G, Reinecke V, Hüdepohl G, Seeber S

出版信息

Int J Radiat Biol Relat Stud Phys Chem Med. 1977 Sep;32(3):229-36. doi: 10.1080/09553007714550941.

PMID:200581

Abstract

The effects of 300 kV X-rays, 60Co gamma-rays, 43 MV X-rays and 43 MeV electrons on total cell RNA and 45 S pre-r RNA were investigated in Novikoff Hepatoma ascites cells. Six hours after local irradiation of the animals with 600 rad, the tumour cells were labelled in vivo for 30 and 60 min. The specific activities of high-molecular-weight RNA were influenced differently. This may be due to divergent effects of the applied radiation qualities at the level of transcription and transformation (processing). In addition, these radiation effects are mediated by the adrenal glands in a rather complex manner. Therefore, the results presented in this study support the suggestion that an in vivo labelling system is unsuitable for the evaluation of quantitative radiation effects on RNA.

摘要

研究了300 kV X射线、60Co γ射线、43 MV X射线和43 MeV电子对诺维科夫肝癌腹水细胞中总细胞RNA和45 S前体rRNA的影响。在用600拉德对动物进行局部照射6小时后，在体内对肿瘤细胞进行30分钟和60分钟的标记。高分子量RNA的比活性受到不同的影响。这可能是由于所应用的辐射质量在转录和转化（加工）水平上的不同作用。此外，这些辐射效应以相当复杂的方式由肾上腺介导。因此，本研究给出的结果支持这样的观点，即体内标记系统不适用于评估对RNA的定量辐射效应。