Department of Medical Chemistry and Biochemistry, Palacky University, Hnevotinska 3, 775 15 Olomouc, Czech Republic.
J Agric Food Chem. 2010 Feb 10;58(3):1672-8. doi: 10.1021/jf903710y.
Cranberry (Vaccinium macrocarpon Ait. Ericaceae) fruits and juice are widely used for their antiadherence and antioxidative properties. Little is known however about their effects on clinical chemistry markers after long-term consumption. This study was conducted to evaluate the effect of three commercial cranberry products, NUTRICRAN90S, HI-PAC 4.0, and PACRAN on the antioxidative status of rodents, divided into three experimental groups. The products were given as dietary admixtures (1500 mg of product/kg of stock feed) for 14 weeks to male Wistar rats (Groups 2-4) and a control Group 1 which received only stock feed. There were no significant cranberry treatment-related effects on oxidative stress parameters, catalase, glutathione peroxidase, glutathione reductase, glutathione transferase, superoxide dismutase, total antioxidant capacity, thiobarbituric acid reactive substances, advanced oxidation protein products, total SH-groups, or any other measured clinical chemistry markers. Hematological parameters, body weight, and food consumption were also unaffected by intake of cranberries. Only liver glutathione reductase activity and glutathione levels were significantly lower in Group 4 than in Group 1. Plasma alkaline phosphatase alone was significantly decreased in Group 2. No gross pathology, effects on organ weights, or histopathology were observed. No genotoxicity was found, and total cytochrome P450 level in liver was unaffected in all groups. The levels of hippuric acid and several phenolic acids were significantly increased in plasma and urine in Groups 2-4. The concentration of anthocyanins was under the detection threshold. The dietary addition of cranberry powders for 14 weeks was well tolerated, but it did not improve the antioxidative status in rats.
蔓越莓（Vaccinium macrocarpon Ait. 杜鹃花科）的果实和果汁因其抗粘附和抗氧化特性而被广泛使用。然而，关于长期食用后对临床化学标志物的影响知之甚少。本研究旨在评估三种商业蔓越莓产品（NUTRICRAN90S、HI-PAC 4.0 和 PACRAN）对啮齿动物抗氧化状态的影响，将它们分为三个实验组。将产品作为饮食添加剂（1500 毫克产品/公斤饲料）给予雄性 Wistar 大鼠（第 2-4 组）和仅接受饲料的对照组（第 1 组）14 周。氧化应激参数、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、谷胱甘肽转移酶、超氧化物歧化酶、总抗氧化能力、硫代巴比妥酸反应物质、高级氧化蛋白产物、总 SH 基团或任何其他测量的临床化学标志物均与蔓越莓处理无关。红细胞参数、体重和食物消耗也不受蔓越莓摄入的影响。只有第 4 组的肝谷胱甘肽还原酶活性和谷胱甘肽水平明显低于第 1 组。只有第 2 组的血浆碱性磷酸酶显著降低。未观察到大体病理学、器官重量变化或组织病理学变化。未发现遗传毒性，所有组的肝总细胞色素 P450 水平均不受影响。血浆和尿液中 hippuric 酸和几种酚酸的水平在第 2-4 组中显著升高。花青素的浓度低于检测阈值。14 周的蔓越莓粉饮食添加被很好地耐受，但不能改善大鼠的抗氧化状态。
