Department of Toxicology, Poznan University of Medical Sciences, Dojazd 30, 60-631 Poznań, Poland.
Department of Pharmacognosy, Poznan University of Medical Sciences, Święcickiego 4, 60-781 Poznań, Poland.
Nutrients. 2021 Mar 24;13(4):1050. doi: 10.3390/nu13041050.
The high polyphenols content of cranberry accounts for its strong antioxidant activity underlying the beneficial health effects of this fruit. Rotenone (ROT) is a specific inhibitor of mitochondrial complex I in the brain which leads to the generation of oxidative stress. To date, there are few data indicating that toxicity of ROT is not limited to the brain but can also affect other tissues. We aimed to examine whether ROT-induced oxidative stress could be counteracted by cranberry juice not only in the brain but also in the liver and kidney. Wistar rats were given the combined treatment with ROT and cranberry juice (CJ) for 35 days. Parameters of antioxidant status were determined in the organs. ROT enhanced lipid peroxidation solely in the brain. The increase in the DNA damage was noticed in all organs examined and in leukocytes. The beneficial effect of CJ on these parameters appeared only in the brain. Additionally, CJ decreased the activity of serum hepatic enzymes. The effect of CJ on antioxidant enzymes was not consistent, however, in some organs, CJ reversed changes evoked by ROT. Summing up, ROT can cause oxidative damage not only in the brain but also in other organs. CJ demonstrated a protective effect against ROT-induced toxicity.
蔓越莓中的高多酚含量解释了其强大的抗氧化活性，这是这种水果对健康有益的原因。鱼藤酮（ROT）是一种特异性的线粒体复合物 I 抑制剂，会导致氧化应激的产生。迄今为止，很少有数据表明 ROT 的毒性不仅局限于大脑，还会影响其他组织。我们旨在研究 ROT 诱导的氧化应激是否不仅可以在大脑中，而且可以在肝脏和肾脏中被蔓越莓汁抵消。Wistar 大鼠接受 ROT 和蔓越莓汁（CJ）联合治疗 35 天。在器官中测定抗氧化状态参数。ROT 仅在大脑中增强了脂质过氧化。在所有检查的器官和白细胞中均发现 DNA 损伤增加。CJ 对这些参数的有益影响仅在大脑中出现。此外，CJ 降低了血清肝酶的活性。然而，CJ 对抗氧化酶的作用并不一致，在某些器官中，CJ 逆转了 ROT 引起的变化。总之，ROT 不仅会在大脑中，而且会在其他器官中引起氧化损伤。CJ 对 ROT 诱导的毒性表现出保护作用。
