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血小板衍生生长因子可增强磷脂酶C-γ1和磷脂酶C-γ2的体内活性。

Platelet-derived growth factor increases the in vivo activity of phospholipase C-gamma 1 and phospholipase C-gamma 2.

作者信息

Sultzman L, Ellis C, Lin L L, Pawson T, Knopf J

机构信息

Genetics Institute, Cambridge, Massachusetts 02140.

出版信息

Mol Cell Biol. 1991 Apr;11(4):2018-25. doi: 10.1128/mcb.11.4.2018-2025.1991.

DOI:10.1128/mcb.11.4.2018-2025.1991
PMID:2005895
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC359888/
Abstract

Upon binding to its cell surface receptor, platelet-derived growth factor (PDGF) causes the tyrosine phosphorylation of phospholipase C-gamma 1 (PLC-gamma 1) and stimulates the production of diacylglycerol and inositol 1,4,5-triphosphate. We showed that following stimulation by PDGF, rat-2 cells overexpressing PLC-gamma 1 display an increase in the levels of both tyrosine-phosphorylated PLC-gamma 1 and inositol phosphates compared with the parental rat-2 cells. This increased responsiveness to PDGF is a direct effect of PLC-gamma 1 overexpression, as a cell line expressing similar levels of an enzymatically inactive point mutant of PLC-gamma 1, PLC-gamma 1 335Q, did not show elevated inositol phosphate production in response to PDGF. Hematopoietic cells express PLC-gamma 2, a PLC isoform that is closely related to PLC-gamma 1. When rat-2 cells overexpressing PLC-gamma 2 were treated with PDGF, an increase in both the tyrosine phosphorylation and the in vivo activity of PLC-gamma 2 was observed. Aluminum fluoride (AIF4-), a universal activator of PLC linked to G-proteins, did not produce an increase in the levels of inositol phosphates in either of the overexpressing cell lines compared with parental rat-2 cells, demonstrating that PLC-gamma isoforms respond specifically to a receptor with tyrosine kinase activity.

摘要

血小板衍生生长因子（PDGF）与细胞表面受体结合后，会导致磷脂酶C-γ1（PLC-γ1）的酪氨酸磷酸化，并刺激二酰基甘油和肌醇1,4,5-三磷酸的产生。我们发现，在PDGF刺激后，与亲代大鼠-2细胞相比，过表达PLC-γ1的大鼠-2细胞中酪氨酸磷酸化的PLC-γ1和肌醇磷酸的水平均有所增加。对PDGF反应性的增加是PLC-γ1过表达的直接作用，因为表达类似水平的PLC-γ1酶活性无活性点突变体PLC-γ1 335Q的细胞系，对PDGF刺激并未表现出肌醇磷酸生成增加。造血细胞表达PLC-γ2，这是一种与PLC-γ1密切相关的PLC同工型。当用PDGF处理过表达PLC-γ2的大鼠-2细胞时，观察到PLC-γ2的酪氨酸磷酸化和体内活性均增加。与亲代大鼠-2细胞相比，与G蛋白相关的PLC通用激活剂氟化铝（AIF4-）在两种过表达细胞系中均未使肌醇磷酸水平增加，这表明PLC-γ同工型对具有酪氨酸激酶活性的受体有特异性反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/351d/359888/3e90656c3b27/molcellb00138-0258-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/351d/359888/e1736e264441/molcellb00138-0255-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/351d/359888/c8a12e13de34/molcellb00138-0256-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/351d/359888/5d05e7f87c2e/molcellb00138-0256-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/351d/359888/6d78f3f05097/molcellb00138-0256-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/351d/359888/3e90656c3b27/molcellb00138-0258-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/351d/359888/e1736e264441/molcellb00138-0255-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/351d/359888/c8a12e13de34/molcellb00138-0256-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/351d/359888/5d05e7f87c2e/molcellb00138-0256-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/351d/359888/6d78f3f05097/molcellb00138-0256-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/351d/359888/3e90656c3b27/molcellb00138-0258-a.jpg

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本文引用的文献

1
Inositol trisphosphate and diacylglycerol as second messengers.肌醇三磷酸和二酰甘油作为第二信使。
Biochem J. 1984 Jun 1;220(2):345-60. doi: 10.1042/bj2200345.
2
Molecular cloning and complete amino-acid sequence of form-I phosphoinositide-specific phospholipase C.I型磷酸肌醇特异性磷脂酶C的分子克隆及完整氨基酸序列
Nature. 1988 Jul 21;334(6179):268-70. doi: 10.1038/334268a0.
3
Cloning and sequence of multiple forms of phospholipase C.磷脂酶C多种形式的克隆与序列分析
Membrane environment exerts an important influence on rac-mediated activation of phospholipase Cγ2.
膜环境对 rac 介导的磷酯酶 Cγ2 的激活有重要影响。
Mol Cell Biol. 2011 Mar;31(6):1240-51. doi: 10.1128/MCB.01408-10. Epub 2011 Jan 18.
4
Control of oligodendrocyte generation and proliferation by Shp2 protein tyrosine phosphatase.Shp2 蛋白酪氨酸磷酸酶对少突胶质细胞生成和增殖的调控。
Glia. 2010 Sep;58(12):1407-14. doi: 10.1002/glia.21016.
5
PLCgamma2 Activates CREB-dependent Transcription in PC12 Cells Through Phosphorylation of CREB at Serine 133.PLCγ2 通过磷酸化 CREB 丝氨酸 133 激活 PC12 细胞中的 CREB 依赖性转录。
Cytotechnology. 2005 Jan;47(1-3):107-16. doi: 10.1007/s10616-005-3763-6.
6
Real time fluorescence imaging of PLC gamma translocation and its interaction with the epidermal growth factor receptor.PLCγ转位及其与表皮生长因子受体相互作用的实时荧光成像
J Cell Biol. 2001 Apr 30;153(3):599-612. doi: 10.1083/jcb.153.3.599.
7
Epidermal growth factor signaling and mitogenesis in Plcg1 null mouse embryonic fibroblasts.磷脂酶Cγ1基因敲除小鼠胚胎成纤维细胞中的表皮生长因子信号传导与有丝分裂
Mol Biol Cell. 1998 Apr;9(4):749-57. doi: 10.1091/mbc.9.4.749.
8
Intimin-dependent binding of enteropathogenic Escherichia coli to host cells triggers novel signaling events, including tyrosine phosphorylation of phospholipase C-gamma1.肠道致病性大肠杆菌对宿主细胞的紧密素依赖性结合引发了新的信号转导事件，包括磷脂酶C-γ1的酪氨酸磷酸化。
Infect Immun. 1997 Jul;65(7):2528-36. doi: 10.1128/iai.65.7.2528-2536.1997.
9
Essential role of the tyrosine kinase substrate phospholipase C-gamma1 in mammalian growth and development.酪氨酸激酶底物磷脂酶C-γ1在哺乳动物生长发育中的重要作用。
Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):2999-3003. doi: 10.1073/pnas.94.7.2999.
10
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Mol Biol Cell. 1993 Jan;4(1):49-57. doi: 10.1091/mbc.4.1.49.
Cell. 1988 Jul 15;54(2):161-9. doi: 10.1016/0092-8674(88)90548-x.
4
Inositol trisphosphate and diacylglycerol: two interacting second messengers.肌醇三磷酸和二酰甘油：两种相互作用的第二信使。
Annu Rev Biochem. 1987;56:159-93. doi: 10.1146/annurev.bi.56.070187.001111.
5
Phospholipase C-148: chromosomal location and deletion mapping of functional domains.
Cold Spring Harb Symp Quant Biol. 1988;53 Pt 2:915-20. doi: 10.1101/sqb.1988.053.01.105.
6
Non-catalytic domains of cytoplasmic protein-tyrosine kinases: regulatory elements in signal transduction.细胞质蛋白酪氨酸激酶的非催化结构域：信号转导中的调节元件
Oncogene. 1988 Nov;3(5):491-5.
7
Signal transduction from membrane to cytoplasm: growth factors and membrane-bound oncogene products increase Raf-1 phosphorylation and associated protein kinase activity.从细胞膜到细胞质的信号转导：生长因子和膜结合癌基因产物增加Raf-1磷酸化及相关蛋白激酶活性。
Proc Natl Acad Sci U S A. 1988 Dec;85(23):8855-9. doi: 10.1073/pnas.85.23.8855.
8
A noncatalytic domain conserved among cytoplasmic protein-tyrosine kinases modifies the kinase function and transforming activity of Fujinami sarcoma virus P130gag-fps.在细胞质蛋白酪氨酸激酶中保守的一个非催化结构域可改变藤浪肉瘤病毒P130gag-fps的激酶功能和转化活性。
Mol Cell Biol. 1986 Dec;6(12):4396-408. doi: 10.1128/mcb.6.12.4396-4408.1986.
9
A large region (approximately equal to 95 kDa) of human factor VIII is dispensable for in vitro procoagulant activity.人凝血因子 VIII 的一个大片段（约相当于 95 kDa）对于体外促凝血活性而言并非必需。
Proc Natl Acad Sci U S A. 1986 Aug;83(16):5939-42. doi: 10.1073/pnas.83.16.5939.
10
Complete cDNA encoding a putative phospholipase C from transformed human lymphocytes.编码来自转化人淋巴细胞的一种假定磷脂酶C的完整互补DNA。
FEBS Lett. 1988 Dec 19;242(1):31-5. doi: 10.1016/0014-5793(88)80979-7.