University of Leipzig, Department for Dermatology, Venerology, and Allergology, Philipp-Rosenthal-Strasse 23, Leipzig 04103 Germany.
J Biomed Opt. 2009 Nov-Dec;14(6):064044. doi: 10.1117/1.3275479.
Despite growing clinical evidence of ablative fractional photothermolysis (AFP), little is known about the spatiotemporal molecular changes within the targeted compartments. Six subjects received three different single AFP treatments using a scanned 250 mum CO(2)-laser beam. Spatiotemporal changes of skin regeneration were estimated by immunohistochemical investigation (HSP70, HSP72, HSP47, TGFbeta, procollagen III, CD3, CD20, and CD68) in skin samples 1 h, 3 days, and 14 days postintervention. The remodeling was uniformly started by regrowth of the epidermal compartment followed by partial to complete replacement of the microscopic ablation zones (MAZ) by newly synthesized condensed procollagen III. From day 3 to 14, the number of macrophages as well as giant cells surrounding the MAZ increased. TGFbeta expression was highest 1 h to 3 days following AFP. HSP70 and HSP72 expressions were highest 3-14 days postintervention in the spinocellular layer leading to an upregulation of HSP47. AFP performed by a scanned CO(2)-laser results in an early epidermal remodeling, which is followed by a dermal remodeling leading to a replacement of the MAZ with newly synthesized (pro)-collagen. During this, an inflammatory infiltrate with CD3(+) and CD20(+) cells surrounds the MAZ. The count of macrophages and giant cells involved in the replacement of the necrotic zones seems to be crucial for wound healing.
尽管烧蚀性分数光热解（AFP）的临床证据不断增加，但对于靶位隔室中分子的时空变化知之甚少。六位受试者接受了三种不同的单次 AFP 治疗，使用扫描的 250 微米 CO2 激光束。通过免疫组织化学研究（HSP70、HSP72、HSP47、TGFβ、前胶原蛋白 III、CD3、CD20 和 CD68），在干预后 1 小时、3 天和 14 天估计皮肤再生的时空变化。重塑是通过表皮隔室的再生均匀开始的，随后是通过新合成的浓缩前胶原蛋白 III 对微观消融区（MAZ）的部分到完全替代。从第 3 天到第 14 天，围绕 MAZ 的巨噬细胞和巨细胞数量增加。TGFβ表达在 AFP 后 1 小时至 3 天最高。HSP70 和 HSP72 的表达在干预后 3-14 天在棘细胞层中最高，导致 HSP47 的上调。通过扫描 CO2 激光进行的 AFP 导致早期表皮重塑，随后是真皮重塑，导致 MAZ 被新合成的（原）胶原蛋白替代。在此期间，CD3(+)和 CD20(+)细胞浸润的炎症细胞围绕着 MAZ。参与坏死区替代的巨噬细胞和巨细胞的数量似乎对伤口愈合至关重要。
