Ray A C, Dwyer J N, Reagor J C
J Assoc Off Anal Chem. 1977 Nov;60(6):1296-1301.
Vitamin D3 is determined in livestock feed supplements by high pressure liquid chromatography (HPLC). Extracts of the samples are quantitated using normal phase chromatography. If interfering co-extractives are present, an aliquot of the extract is injected on the normal phase column, and the fraction corresponding to vitamin D3 is collected. The vitamin fraction is then further cleaned up and separated from interferences by reverse phase chromatography, and quantitated by measuring the ultraviolet absorption at 254 and 280 nm. The method measures actual vitamin D3 content in the presence of pre-vitamin D, tachysterol, isotachysterol, and vitamin A.
采用高压液相色谱法（HPLC）测定家畜饲料补充剂中的维生素D3。使用正相色谱法对样品提取物进行定量。如果存在干扰性共提取物，则将一部分提取物注入正相柱，收集与维生素D3相对应的馏分。然后，通过反相色谱法对维生素馏分进一步净化并与干扰物分离，并通过测量254和280nm处的紫外线吸收进行定量。该方法可在存在前维生素D、速甾醇、异速甾醇和维生素A的情况下测定实际的维生素D3含量。
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