高效液相色谱法测定猪饲料及添加剂中的卡巴氧和酒石酸噻嘧啶

High pressure liquid chromatographic determination of carbadox and pyrantel tartrate in swine feed and supplements.

作者信息

Lowie D M, Teague R T, Quick F E, Foster C L

出版信息

J Assoc Off Anal Chem. 1983 May;66(3):602-5.

Abstract

A rapid yet reliable procedure for the simultaneous extraction and assay of carbadox and pyrantel tartrate is described. The feed is extracted with water-acetonitrile-methanol and cleaned up on a short alumina column. The eluant is separated by high pressure liquid chromatography and the compounds are detected at different wavelengths. The drugs of interest are well resolved in all feeds studied. The procedure has also been applied to a wide range of feeds which contained either one of the drugs or both in combination. No significant interferences were observed. Spiked sample recoveries were 97% for carbadox and 101% for pyrantel tartrate. Ruggedness test coefficients of variation were 2.0% for carbadox and 2.1% for pyrantel tartrate.

摘要

本文描述了一种快速且可靠的同时提取和测定卡巴多司及酒石酸噻嘧啶的方法。样品用水 - 乙腈 - 甲醇进行提取，并在短氧化铝柱上进行净化处理。洗脱液通过高压液相色谱进行分离，各化合物在不同波长下进行检测。在所研究的所有样品中，目标药物均能得到良好分离。该方法还应用于多种含有其中一种药物或两种药物组合的样品，未观察到明显干扰。加标样品中卡巴多司的回收率为97%，酒石酸噻嘧啶的回收率为101%。卡巴多司的耐用性测试变异系数为2.0%，酒石酸噻嘧啶的为2.1%。