College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Plant Physiol Biochem. 2010 Feb-Mar;48(2-3):142-52. doi: 10.1016/j.plaphy.2009.12.002. Epub 2009 Dec 17.
Current research indicated that the resveratrol was mainly accumulated in the skin of grape berry, however, little is yet known about the distribution of resveratrol, as well as the regulation mechanism at protein level and the localization of stilbene synthase (malonyl-CoA:4-coumaroyl-CoA malonyltransferase; EC 2.3.1.95; STS), a key enzyme of resveratrol biosynthesis, in young grape plants (Vitis vinifera L. cv. Cabernet Sauvignon). Resveratrol, whose constitutive level ranged from 0.2 mg kg(-1) FW to 16.5 mg kg(-1) FW, could be detected in stem, axillary bud, shoot tip, petiole, root and leaf of grape plants. Among them, stem phloems presented the most abundant of resveratrol, and the leaves presented the lowest. Interestingly, the level of STS mRNA and protein were highest in grape leaves. And the analysis of immunohistochemical showed the tissue-specific distribution of STS in different organs, presenting the similar results compared with the amount of protein. And the subcellular localization revealed that the cell wall in different tissues processed the most golden particles representing STS. Subjecting to UV-C irradiation, resveratrol and STS were both intensely stimulated in grape leaves, with the similar response pattern. Results above indicated that distribution of resveratrol and STS in grape was organ-specific and tissue-specific. And the accumulation of resveratrol induced by UV-C was regulated by transcriptional and translational level of STS.
目前的研究表明，白藜芦醇主要积累在葡萄浆果的果皮中，但关于白藜芦醇的分布、白藜芦醇生物合成的关键酶芪合酶（丙二酰辅酶 A：4-香豆酰辅酶 A 丙二酰转移酶；EC 2.3.1.95；STS）在蛋白质水平上的调控机制以及在幼葡萄植株（Vitis vinifera L. cv. Cabernet Sauvignon）中的定位，人们知之甚少。组成型白藜芦醇水平在 0.2 mg kg(-1) FW 到 16.5 mg kg(-1) FW 之间，可在葡萄植株的茎、腋芽、梢、叶柄、根和叶中检测到。其中，茎韧皮部的白藜芦醇含量最丰富，叶片的含量最低。有趣的是，STS mRNA 和蛋白水平在葡萄叶片中最高。免疫组织化学分析表明 STS 在不同器官中的组织特异性分布与蛋白含量的结果相似。亚细胞定位显示不同组织的细胞壁处理了最多的 STS 金颗粒。经 UV-C 照射后，葡萄叶片中的白藜芦醇和 STS 均受到强烈刺激，反应模式相似。以上结果表明，白藜芦醇和 STS 在葡萄中的分布具有器官特异性和组织特异性。UV-C 诱导的白藜芦醇积累受 STS 的转录和翻译水平调控。
