Heraud Philip, Ng Elizabeth S, Caine Sally, Yu Qing C, Hirst Claire, Mayberry Robyn, Bruce Amanda, Wood Bayden R, McNaughton Don, Stanley Edouard G, Elefanty Andrew G
Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria 3800, Australia.
Stem Cell Res. 2010 Mar;4(2):140-7. doi: 10.1016/j.scr.2009.11.002. Epub 2009 Dec 2.
Human ESCs (hESCs) are a valuable tool for the study of early human development and represent a source of normal differentiated cells for pharmaceutical and biotechnology applications and ultimately for cell replacement therapies. For all applications, it will be necessary to develop assays to validate the efficacy of hESC differentiation. We explored the capacity for FTIR spectroscopy, a technique that rapidly characterises cellular macromolecular composition, to discriminate mesendoderm or ectoderm committed cells from undifferentiated hESCs. Distinct infrared spectroscopic "signatures" readily distinguished hESCs from these early differentiated progeny, with bioinformatic models able to correctly classify over 97% of spectra. These data identify a role for FTIR spectroscopy as a new modality to complement conventional analyses of hESCs and their derivatives. FTIR spectroscopy has the potential to provide low-cost, automatable measurements for the quality control of stem and differentiated cells to be used in industry and regenerative medicine.
人类胚胎干细胞（hESCs）是研究人类早期发育的宝贵工具，也是用于制药和生物技术应用乃至细胞替代疗法的正常分化细胞来源。对于所有应用而言，开发检测方法以验证hESC分化的有效性将是必要的。我们探索了傅里叶变换红外光谱（FTIR）技术区分中内胚层或外胚层定向分化细胞与未分化hESCs的能力，该技术可快速表征细胞大分子组成。独特的红外光谱“特征”能够轻松区分hESCs与这些早期分化后代，生物信息学模型能够正确分类超过97%的光谱。这些数据表明FTIR光谱可作为一种新方法，补充对hESCs及其衍生物的传统分析。FTIR光谱有潜力为工业和再生医学中使用的干细胞及分化细胞的质量控制提供低成本、可自动化的测量。
