Synchrotron Light Research Institute, Muang, Nakhon Ratchasima 30000, Thailand.
J Biomed Opt. 2011 May;16(5):057005. doi: 10.1117/1.3580253.
Stem cell-based therapy for liver regeneration has been proposed to overcome the persistent shortage in the supply of suitable donor organs. A requirement for this to succeed is to find a rapid method to detect functional hepatocytes, differentiated from embryonic stem cells. We propose Fourier transform infrared (FTIR) microspectroscopy as a versatile method to identify the early and last stages of the differentiation process leading to the formation of hepatocytes. Using synchrotron-FTIR microspectroscopy, the means of identifying hepatocytes at the single-cell level is possible and explored. Principal component analysis and subsequent partial least-squares (PLS) discriminant analysis is applied to distinguish endoderm induction from hepatic progenitor cells and matured hepatocyte-like cells. The data are well modeled by PLS with endoderm induction, hepatic progenitor cells, and mature hepatocyte-like cells able to be discriminated with very high sensitivity and specificity. This method provides a practical tool to monitor endoderm induction and has the potential to be applied for quality control of cell differentiation leading to hepatocyte formation.
基于干细胞的肝脏再生治疗方法被提出以克服合适供体器官持续短缺的问题。要使这一方法成功，就需要找到一种快速的方法来检测从胚胎干细胞分化而来的功能性肝细胞。我们提出傅里叶变换红外（FTIR）微光谱法作为一种通用的方法来鉴定导致肝细胞形成的早期和最后阶段的分化过程。使用同步加速器-FTIR 微光谱法，可以在单细胞水平上识别肝细胞的方法进行了探索。主成分分析和随后的偏最小二乘（PLS）判别分析被应用于区分内胚层诱导和肝祖细胞以及成熟的类肝细胞。PLS 很好地对数据进行了建模，能够非常高的灵敏度和特异性区分内胚层诱导、肝祖细胞和成熟的类肝细胞。这种方法为监测内胚层诱导提供了一种实用的工具，并且有可能应用于细胞分化以形成肝细胞的质量控制。
