Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Nanomedicine. 2010 Aug;6(4):570-4. doi: 10.1016/j.nano.2009.12.002. Epub 2010 Jan 7.
Concern is continuously raised regarding the safety of preservatives, which are crucial in most cosmetic preparations. The antimicrobial effects of silver (Ag) are well recognized; however, Ag has some limitations as a preservative, such as its interference with salts. In this study, we investigated the effects of recently synthesized Ag nanoparticles on microorganisms, the permeability of Ag nanoparticles in human skin, and the cytotoxicity of Ag nanoparticles in human keratinocytes under ultraviolet B irradiation. Ag nanoparticles were found to be very stable, and they did not exhibit sedimentation for over 1 year. Ag nanoparticles showed sufficient preservation efficacy against mixed bacteria and mixed fungi, and did not penetrate normal human skin. At concentrations of 0.002-0.02 parts per million, Ag nanoparticles had no effect on HaCaT keratinocytes and did not enhance ultraviolet B-induced cell death. These results suggest that Ag nanoparticles may have potential for use as a preservative in cosmetics. From the clinical editor: In this study, the effects of recently synthesized Ag nanoparticles were investigated on microorganisms, along with the skin permeability and the cytotoxicity in human keratinocytes under UVB-irradiation. Ag nanoparticles were found to be very stable, showed sufficient preservation efficacy against mixed bacteria and mixed fungi, and did not penetrate normal human skin. Ag nanoparticles appear to be suitable for use as a preservative in cosmetics.
人们一直对防腐剂的安全性表示担忧，因为防腐剂在大多数化妆品制剂中都是至关重要的。银（Ag）的抗菌效果是众所周知的；然而，Ag 作为防腐剂有一些局限性，例如它会干扰盐类。在这项研究中，我们研究了最近合成的 Ag 纳米粒子对微生物的影响、Ag 纳米粒子在人体皮肤中的渗透性以及在紫外线 B 照射下 Ag 纳米粒子对人角质形成细胞的细胞毒性。Ag 纳米粒子非常稳定，在超过 1 年的时间里没有出现沉淀。Ag 纳米粒子对混合细菌和混合真菌具有足够的保存效果，并且不会穿透正常的人体皮肤。在 0.002-0.02ppm 的浓度下，Ag 纳米粒子对 HaCaT 角质形成细胞没有影响，也不会增强紫外线 B 诱导的细胞死亡。这些结果表明，Ag 纳米粒子可能有潜力用作化妆品中的防腐剂。从临床编辑的角度来看：在这项研究中，研究了最近合成的 Ag 纳米粒子对微生物的影响，以及在紫外线 B 照射下 Ag 纳米粒子在人体角质形成细胞中的皮肤渗透性和细胞毒性。Ag 纳米粒子非常稳定，对混合细菌和混合真菌具有足够的保存效果，并且不会穿透正常的人体皮肤。Ag 纳米粒子似乎适合用作化妆品中的防腐剂。
