Marinas Ioana C, Ignat Leonard, Maurușa Ignat E, Gaboreanu Madalina D, Adina Coroabă, Popa Marcela, Chifiriuc Mariana C, Angheloiu Marian, Georgescu Mihaela, Iacobescu Alexandra, Pircalabioru Gratiela Gradisteanu, Stan Miruna, Pinteala Mariana
Research Institute of the University of Bucharest-ICUB, University of Bucharest, 050095, Bucharest, Romania.
Research and Development Department of SC Sanimed International Impex SRL, 6 Bucharest -Giurgiu Street, 087040, Giurgiu, Romania.
Heliyon. 2024 Feb 9;10(4):e26047. doi: 10.1016/j.heliyon.2024.e26047. eCollection 2024 Feb 29.
Chronic wounds represent one of the complications that might occur from the disruption of wound healing process. Recently, there has been a rise in interest in employing nanotechnology to develop novel strategies for accelerating wound healing. The aim of the present study was to use a green synthesis method to obtain AgNPs/NaLS systems useful for wounds management and perform an in-depth investigation of their behavior during and post-synthesis as well as of their biological properties. The colloids obtained from silver nanoparticles (AgNPs) and commercial sodium lignosulfonate (NaLS) in a single-pot aqueous procedure have been fully characterized by UV-Vis, FT-IR, DLS, TEM, XRD, and XPS to evaluate the synthesis efficiency and to provide new insights in the process of AgNPs formation and NaLS behavior in aqueous solutions. The effects of various concentrations of NaLS (0-16 mg/mL) and AgNO (0-20 mM) and of two different temperatures on AgNPs formation have been analyzed. Although the room temperature is feasible for AgNPs synthesis, the short mixing at 70 °C significantly increases the speed of nanoparticle formation and storage stability. In all experimental conditions AgNPs of 20-40 nm in size have been obtained. The antimicrobial activity assessed quantitatively on clinical and reference bacterial strains, both in suspension and biofilm growth state, revealed a broad antimicrobial spectrum, the most intensive inhibitory effect being noticed against and strains. The AgNP/NaLS enhanced the NO extracellular release, potentially contributing to the microbicidal and anti-adherence activity by protein oxidation. Both AgNP/NaLS and NaLS were non-hemolytic (hemolytic index<5%, 2.26 ± 0.13% hemolysis) and biocompatible (102.17 ± 3.43 % HaCaT cells viability). The presence of AgNPs increased the antioxidative activity and induced a significant cytotoxicity on non-melanoma skin cancer cells (62.86 ± 8.27% Cal-27 cells viability). Taken together, all these features suggest the multivalent potential of these colloids for the development of novel strategies for wound management, acting by preventing infection-associated complications and supporting the tissue regeneration.
慢性伤口是伤口愈合过程中断可能引发的并发症之一。近来，利用纳米技术开发加速伤口愈合新策略的兴趣日益浓厚。本研究的目的是采用绿色合成方法获得可用于伤口处理的AgNPs/NaLS体系，并对其合成过程中及合成后的行为及其生物学特性进行深入研究。通过紫外可见光谱（UV-Vis）、傅里叶变换红外光谱（FT-IR）、动态光散射（DLS）、透射电子显微镜（TEM）、X射线衍射（XRD）和X射线光电子能谱（XPS）对在单锅水相过程中由银纳米颗粒（AgNPs）和商业木质素磺酸钠（NaLS）获得的胶体进行了全面表征，以评估合成效率，并为AgNPs形成过程和NaLS在水溶液中的行为提供新的见解。分析了不同浓度的NaLS（0 - 16 mg/mL）和AgNO₃（0 - 20 mM）以及两种不同温度对AgNPs形成的影响。虽然室温对于AgNPs合成是可行的，但在70℃下短时间混合显著提高了纳米颗粒的形成速度和储存稳定性。在所有实验条件下均获得了尺寸为20 - 40 nm的AgNPs。对临床和参考细菌菌株在悬浮液和生物膜生长状态下进行的定量抗菌活性评估显示出广泛的抗菌谱，对[具体菌株1]和[具体菌株2]菌株的抑制作用最为强烈。AgNP/NaLS增强了细胞外NO释放，可能通过蛋白质氧化有助于杀菌和抗粘附活性。AgNP/NaLS和NaLS均无溶血作用（溶血指数<5%，溶血率为2.26±0.13%）且具有生物相容性（HaCaT细胞活力为102.17±3.
