Laboratorio de Ecoloxía Mariña, Departamento de Ecoloxía e Bioloxía Animal, Facultade de Ciencias do Mar, Universidade de Vigo, Estda. Colexio universitario s/n, 36310 Vigo (Galicia), Spain.
Ecotoxicol Environ Saf. 2010 Mar;73(3):254-61. doi: 10.1016/j.ecoenv.2009.11.010. Epub 2010 Jan 8.
Laboratory cultures of the flagellate Isochrysis galbana and a coastal phytoplankton assemblage were used to assess the feasibility of variable fluorescence (F(v)), measured by means of fast repetition rate fluorometry, as endpoint to monitor the effect of fuel on microalgae as compared to phytoplankton growth rate. Parallel changes in cell size of I. galbana and taxonomic composition of the natural assemblage were recorded. The results presented in this investigation indicate that F(v) is a suitable endpoint to this aim, due to the ease and rapidity of the measurement, ecological relevance and sensitivity. The derived effective concentrations provided toxicity thresholds similar to, or even lower than concentrations derived from embryo-larval bioassays with marine organisms. The lowest calculated EC(10) corresponded to that of the natural assemblage, 2.5 microg eq chrysene L(-1), concentration easily exceeded in high maritime traffic areas subjected to frequent spillages.
利用鞭毛藻等鞭毛藻和沿海浮游植物组合的实验室培养物，评估通过快速重复率荧光测量法测量的可变荧光（F(v)）作为监测燃料对微藻影响的终点的可行性，与浮游植物生长率相比。记录了等鞭毛藻的细胞大小和自然组合的分类组成的平行变化。本研究的结果表明，由于测量的简便性和快速性、生态相关性和敏感性，F(v)是一个合适的终点。得出的有效浓度提供了与海洋生物胚胎-幼虫生物测定得出的浓度相似甚至更低的毒性阈值。计算出的最低 EC(10)值对应于自然组合，即 2.5 微克当量芘每升（L(-1)），在经常发生溢油事故的高海上交通区域，很容易超过该浓度。
