Wang Li, Ying Bo, E Xue-li
Institute of Environmental Health and Related Product Safety, Chinese Center for Disease Prevention and Control, Beijing 100021, China.
Wei Sheng Yan Jiu. 2006 Mar;35(2):254-6.
A new type of dual-channel PAM fluorometer (ToxY-PAM) was developed for highly sensitive detection of toxic substances in water samples. In conjunction with suspension of unicellular algae as the biotest material, the ToxY-PAM provided a highly rapid and sensitive bioassay test system for the detection of toxic substances in water samples, which could directly or indirectly produce the inhibition of photosynthetic activity in algae. The development process of the detection of toxic substances in water with fluorescence technique, the basic principle and operation of the ToxY-PAM were reviewed in this paper.
为了高灵敏度地检测水样中的有毒物质，开发了一种新型双通道脉冲幅度调制荧光计（ToxY-PAM）。结合单细胞藻类悬浮液作为生物测试材料，ToxY-PAM为检测水样中的有毒物质提供了一种高度快速且灵敏的生物测定测试系统，这些有毒物质可直接或间接抑制藻类的光合活性。本文综述了利用荧光技术检测水中有毒物质的发展过程、ToxY-PAM的基本原理及操作。
