Chemistry Department, Jinan University, Guangzhou 510632, PR China.
Micron. 2010 Apr;41(3):257-62. doi: 10.1016/j.micron.2009.08.011. Epub 2009 Dec 3.
The mechanical properties of cells are important for many cellular processes. Here, atomic force microscopy (AFM) and laser scanning confocal microscopy (LSCM) were carried out to characterize lymphocyte and Jurkat cells. The average elastic modulus of lymphocyte is 1.24 +/- 0.09 kPa, which is almost twofold higher than that of Jurkat cell (0.51 +/- 0.06 kPa). LSCM images of sub-membrane cytoskeleton showed a significant difference in the organization of their F-actin structures. Lymphocyte cells had more and thicker actin bundles than that of Jurkat cells. Lymphocyte and Jurkat cells after adding the F-actin destabilizing agent Cytochalasin-B (Cyt-B) were also investigated by AFM. A decrease in the elastic modulus of lymphocyte from a value of 1.24 +/- 0.09 kPa down to 0.34 +/- 0.04 kPa for 24 h was observed, and that of Jurkat cell decreased from 0.51 +/- 0.06 kPa to 0.23 +/- 0.04 kPa. We really believe that this technology will be used for cancer detection and opens a door to study the biophysical properties of signaling domains extending from the cell surface to deeper parts of the cell.
细胞的力学特性对于许多细胞过程都很重要。在这里，我们使用原子力显微镜（AFM）和激光共聚焦显微镜（LSCM）来表征淋巴细胞和 Jurkat 细胞。淋巴细胞的平均弹性模量为 1.24 +/- 0.09 kPa，几乎是 Jurkat 细胞（0.51 +/- 0.06 kPa）的两倍。亚细胞膜细胞骨架的 LSCM 图像显示，它们的 F-肌动蛋白结构的组织存在显著差异。淋巴细胞中的肌动蛋白束比 Jurkat 细胞更多且更厚。通过 AFM 还研究了添加 F-肌动蛋白解聚剂细胞松弛素-B（Cyt-B）后淋巴细胞和 Jurkat 细胞的变化。在 24 小时内，淋巴细胞的弹性模量从 1.24 +/- 0.09 kPa 降低到 0.34 +/- 0.04 kPa，而 Jurkat 细胞的弹性模量从 0.51 +/- 0.06 kPa 降低到 0.23 +/- 0.04 kPa。我们真的相信这项技术将用于癌症检测，并为研究从细胞表面延伸到细胞更深部位的信号域的生物物理特性开辟了一扇门。
