Cai Mingjun, Wang Huili, Zhao Guanfang, Li Hongru, Gao Jing, Wang Hongda
University of Science and Technology of China, Hefei 230027, China.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Biophys Rep. 2022 Aug 31;8(4):183-192. doi: 10.52601/bpr.2022.220004.
A major role of cell membranes is to provide an ideal environment for the constituent proteins to perform their biological functions. A deep understanding of the membrane proteins assembly process under physiological conditions is quite important to elucidate both the structure and the function of the cell membranes. Along these lines, in this work, a complete workflow of the cell membrane sample preparation and the correlated AFM and dSTORM imaging analysis methods are presented. A specially designed, angle-controlled sample preparation device was used to prepare the cell membrane samples. The correlated distributions of the specific membrane proteins with the topography of the cytoplasmic side of the cell membranes can be obtained by performing correlative AFM and dSTORM measurements. These methods are ideal for systematically studying the structure of the cell membranes. The proposed method of the sample characterization was not only limited to the measurement of the cell membrane but also can be applied for both biological tissue section analysis and detection.
细胞膜的一个主要作用是为组成蛋白提供一个理想的环境，使其能够执行生物学功能。深入了解生理条件下膜蛋白的组装过程对于阐明细胞膜的结构和功能非常重要。沿着这些思路，在本研究中，我们展示了细胞膜样品制备的完整流程以及相关的原子力显微镜（AFM）和直接随机光学重建显微镜（dSTORM）成像分析方法。我们使用了一种专门设计的、角度可控的样品制备装置来制备细胞膜样品。通过进行相关的AFM和dSTORM测量，可以获得特定膜蛋白与细胞膜细胞质侧拓扑结构的相关分布。这些方法对于系统研究细胞膜结构来说是理想的。所提出的样品表征方法不仅限于细胞膜的测量，还可应用于生物组织切片分析和检测。
