Preedy Emily Callard, Perni Stefano, Prokopovich Polina
School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, UK. Email:
RSC Adv. 2015 Nov 5;5(102):83885-83898. doi: 10.1039/c5ra15390e. Epub 2015 Oct 2.
Periprosthetic osteolysis and implant loosening are the outcomes of wear debris generation in total joint replacements. Wear debris formed from the implanted materials consisting of metals, polymers, ceramic and bone cement initiate the immune system response. Often osteoblasts, the principal cell type in bone tissue adjacent to the prostheses, are directly impacted. In this study, the influence of cobalt, titanium and PMMA bone cement particles of different sizes, charges and compositions on mouse osteoblast adhesion, nanomechanics (elasticity and spring constant) and metabolic activity were investigated. These studies were accompanied by osteoblast mineralisation experiments and cell uptake after exposure to particles at defined time points. Our results demonstrate that alteration of the nanomechanical properties are mainly dependent on the metal type rather than nanoparticles size and concentration. Moreover, despite uptake increasing over exposure time, the cell characteristics exhibit changes predominately after the first 24 hours, highlighting that the cell responses to nanoparticle exposure are not cumulative. Understanding these processes is critical to expanding our knowledge of implant loosening and elucidating the nature of prosthetic joint failure.
假体周围骨溶解和植入物松动是全关节置换中磨损颗粒产生的后果。由金属、聚合物、陶瓷和骨水泥组成的植入材料形成的磨损颗粒引发免疫系统反应。通常，假体周围骨组织中的主要细胞类型成骨细胞会受到直接影响。在本研究中，研究了不同尺寸、电荷和组成的钴、钛和聚甲基丙烯酸甲酯骨水泥颗粒对小鼠成骨细胞黏附、纳米力学（弹性和弹簧常数）和代谢活性的影响。这些研究还包括成骨细胞矿化实验以及在特定时间点暴露于颗粒后的细胞摄取情况。我们的结果表明，纳米力学性能的改变主要取决于金属类型，而非纳米颗粒的大小和浓度。此外，尽管摄取量随暴露时间增加，但细胞特性主要在最初24小时后出现变化，这突出表明细胞对纳米颗粒暴露的反应并非累积性的。了解这些过程对于扩展我们对植入物松动的认识以及阐明人工关节失效的本质至关重要。
