Vascular Research Unit, Department of Vascular Surgery, Regional Hospital Viborg, Viborg, Denmark.
Eur J Vasc Endovasc Surg. 2010 Apr;39(4):472-7. doi: 10.1016/j.ejvs.2009.11.023. Epub 2010 Jan 8.
To evaluate the efficacy of a silver-coated vascular polyester graft in the prevention of graft infection after inoculation with Staphylococcus aureus in a porcine model.
Eighty-four pigs were randomly selected 1:1 to receive a silver-coated or non-silver-coated 8-mm-wide polyester graft implanted end-to-end in the infrarenal aorta. At the end of implantation, 10(6) colony forming units (CFUs) S. aureus in 0.3ml suspension were inoculated directly on the graft surface. Blood samples assayed for white blood corpuscles (WBCs) and C-reactive protein (CRP) were taken before implantation and on the postoperative days 2, 5, 7, 11 and 14. Two weeks after implantation, the perigraft swabs were analysed for S. aureus or contaminants. CFUs of S. aureus were quantified and logarithmised. Student's t-tests, repeated measurement analysis of variance (ANOVA) and chi-square test were employed to compare the two grafts.
All pigs developed graft infection. There were no statistically significant differences between the silver-coated and non-silver-coated grafts in the quantity of S. aureus, macroscopic signs of infection and postoperative changes in the temperature, WBC and CRP.
Silver-coated polyester grafts failed to prevent graft infections after inoculation with 10(6)S. aureus in 0.3ml suspension in a porcine model.
评估一种涂银聚酯移植物在接种金黄色葡萄球菌后预防感染的疗效，该模型为猪模型。
将 84 头猪随机 1:1 分为两组，分别接受涂银或非涂银 8mm 宽聚酯移植物，在肾下主动脉端端植入。植入结束时，将 0.3ml 悬浮液中 10(6)个金黄色葡萄球菌菌落形成单位（CFU）直接接种在移植物表面。在植入前和术后第 2、5、7、11 和 14 天采集白细胞（WBC）和 C 反应蛋白（CRP）的血样进行检测。植入后两周，对移植物周围拭子进行金黄色葡萄球菌或污染物分析。定量并对数化金黄色葡萄球菌的 CFU。采用 Student's t 检验、重复测量方差分析（ANOVA）和卡方检验比较两种移植物。
所有猪均发生移植物感染。在金黄色葡萄球菌数量、感染的宏观征象以及术后体温、WBC 和 CRP 的变化方面，涂银和非涂银聚酯移植物之间无统计学差异。
在猪模型中，涂银聚酯移植物未能预防 10(6)金黄色葡萄球菌 0.3ml 悬浮液接种后的感染。
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