Universidade Federal Fluminense - Departamento de Morfologia - Niterói (RJ) - Brazil.
Universidade de Vassouras - Maricá (RJ) - Brazil.
Acta Cir Bras. 2024 Oct 7;39:e397524. doi: 10.1590/acb397524. eCollection 2024.
To histologically quantify the different tissues that make up the porcine ureter, (epithelial, connective, and muscular tissue) in the three segments labelled: cranial, middle and caudal, in order to identify the segment most compatible for use as a vascular graft.
Fifteen porcine ureters were collected, divided into the three segments, and the samples were stained with hematoxylin and eosin, picrosirius red and Weigert's resorcin-fuchsin. The immunohistochemistry technique was applied for alpha-smooth muscle actin. Collagen fibers, muscle, epithelium, and elastic fibers tissue were quantified, in the entire ureter, and divided into hemispheres, comparing the different segments.
When comparing hemisphere segments, significant differences were observed (p < 0.01) for collagen and muscle tissue, with the cranial segment presenting the greatest amount of these components when compared to the middle and caudal. No significant difference was observed between the segments when comparing the entire ureters.
After comparing the segments by hemisphere, the cranial segment presented a slight advantage for use as a vascular graft due to presenting greater collagen fiber content.
通过组织学方法对猪输尿管的三个节段（头段、中段和尾段）中的不同组织（上皮组织、结缔组织和肌肉组织）进行定量分析，以确定最适合用作血管移植物的节段。
收集 15 根猪输尿管，分为三个节段，用苏木精-伊红、苦味酸-天狼星红和魏格特氏间苯二酚复红染色。应用免疫组织化学技术检测α-平滑肌肌动蛋白。对胶原纤维、肌肉、上皮和弹性纤维组织进行定量分析，比较不同节段的整个输尿管。
对半球节段进行比较时，观察到胶原和肌肉组织存在显著差异（p<0.01），与中、尾段相比，头段的这些成分含量最高。比较整个输尿管时，各节段之间无显著差异。
通过对半球节段进行比较，头段由于胶原纤维含量较高，在用作血管移植物方面略有优势。
