Moore Jennifer C, Sadowy Sasha, Alikani Mina, Toro-Ramos Alana J, Swerdel Mavis R, Hart Ronald P, Cohen Rick I
Stem Cell Research Center, Rutgers the State University of New Jersey, Piscataway, NJ 08854, USA.
Stem Cell Res. 2010 Mar;4(2):92-106. doi: 10.1016/j.scr.2009.11.001. Epub 2009 Nov 24.
Meticulous characterization of human embryonic stem cells (hESC) is critical to their eventual use in cell-based therapies, particularly in view of the diverse methods for derivation and maintenance of these cell lines. However, characterization methods are generally not standardized and many currently used assays are subjective, making dependable and direct comparison of cell lines difficult. In order to address this problem, we selected 10 molecular-based high-resolution assays as components of a panel for characterization of hESC. The selection of the assays was primarily based on their quantitative or objective (rather than subjective) nature. We demonstrate the efficacy of this panel by characterizing 4 hESC lines, derived in two different laboratories using different derivation techniques, as pathogen free, genetically stable, and able to differentiate into derivatives of all three germ layers. Our panel expands and refines a characterization panel previously proposed by the International Stem Cell Initiative and is another step toward standardized hESC characterization and quality control, a crucial element of successful hESC research and clinical translation.
对人类胚胎干细胞（hESC）进行细致的特征描述对于其最终用于基于细胞的治疗至关重要，特别是考虑到这些细胞系的衍生和维持方法多种多样。然而，特征描述方法通常并不标准化，而且目前许多使用的检测方法具有主观性，使得细胞系之间可靠且直接的比较变得困难。为了解决这个问题，我们选择了10种基于分子的高分辨率检测方法作为一组用于hESC特征描述的组成部分。这些检测方法的选择主要基于其定量或客观（而非主观）的性质。我们通过对4种hESC系进行特征描述来证明这一组检测方法的有效性，这4种细胞系是在两个不同实验室使用不同衍生技术获得的，结果表明它们无病原体、基因稳定，并且能够分化为所有三个胚层的衍生物。我们的这一组检测方法扩展并完善了国际干细胞研究倡议组织先前提出的特征描述组，是朝着标准化hESC特征描述和质量控制迈出的又一步，而这是成功进行hESC研究和临床转化的关键要素。
