Department of Feed Science, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnical University, Wuhan, Hubei, China.
Cancer Epidemiol. 2010 Feb;34(1):93-5. doi: 10.1016/j.canep.2009.12.009. Epub 2010 Jan 8.
The -93 SNP of MLH1 gene is associated with MLH1 gene methylation in endometrial and colorectal cancers. We undertook luciferase reporter assay and electrophoretic mobility shift assay (EMSA) to test whether the -93 SNP affects the MLH1 gene expression. The luciferase activity for -93A plasmid is significantly lower than -93G plasmid. In EMSA experiments, the -93A and -93G probes have different binding affinity to nuclear proteins of JEG3 cells. Our data indicate that -93 SNP affects MLH1 gene expression by altering protein binding to the promoter of MLH1 gene.
MLH1 基因的-93 SNP 与子宫内膜癌和结直肠癌中的 MLH1 基因甲基化有关。我们进行了荧光素酶报告基因检测和电泳迁移率变动分析（EMSA），以检测-93 SNP 是否影响 MLH1 基因的表达。-93A 质粒的荧光素酶活性明显低于-93G 质粒。在 EMSA 实验中，-93A 和-93G 探针与 JEG3 细胞的核蛋白具有不同的结合亲和力。我们的数据表明，-93 SNP 通过改变与 MLH1 基因启动子结合的蛋白来影响 MLH1 基因的表达。
