Laboratoire de Pharmacognosie, Institut de Chimie Moléculaire de Reims, CNRS UMR 6229, IFR 53 Biomolécules, Bâtiment 18, BP 1039, 51687 Reims Cedex 2, France.
Phytochemistry. 2010 Mar;71(4):429-34. doi: 10.1016/j.phytochem.2009.11.007. Epub 2010 Jan 12.
Phytochemical investigation of the MeOH extract of the stem bark of Antonia ovata led to the isolation of four triterpenoid saponins, along with eleven known compounds. Their structures were established by extensive 1D and 2D NMR, as well as HR-MS analysis and acid hydrolysis. All isolated saponins contained the same tetrasaccharide chain O-beta-d-xylopyranosyl-(1-->2)-O-beta-d-glucopyranosyl-(1-->3)-O-[beta-d-glucopyranosyl-(1-->2)]-beta-d-glucuropyranoside linked to C-3 of esterified derivatives of R(1)-barrigenol, A(1)-barrigenol, barringtogenol C, or camelliagenin. Biological evaluation of the compounds against KB cell line revealed a potent cytotoxic activity with IC(50) values ranging from 3.1 to 6.6microM. The known compounds were found to be inactive at 10microg/ml concentration.
从桃金娘科植物 Antonia ovata 的茎皮甲醇提取物中分离得到了四种三萜皂苷，以及十一种已知化合物。通过广泛的 1D 和 2D NMR、高分辨率质谱分析和酸水解确定了它们的结构。所有分离得到的皂苷都含有相同的四糖链 O-β-d-吡喃木糖基-(1-->2)-O-β-d-吡喃葡萄糖基-(1-->3)-O-[β-d-吡喃葡萄糖基-(1-->2)]-β-d-吡喃葡萄糖醛酸酯，连接在酯化衍生物的 C-3 上，这些酯化衍生物是由 R(1)-barrigenol、A(1)-barrigenol、barringtogenol C 或 camelliagenin 组成。对这些化合物进行的针对 KB 细胞系的生物活性评价表明，它们具有很强的细胞毒性，IC(50)值范围为 3.1 到 6.6μM。而在 10μg/ml 浓度下，已知化合物则没有活性。
