Das Koushik, Samanta Tanushree Tulsian, Samanta Pradip, Nandi Dilip Kumar
Department of Physiology, Raja N. L. Khan Women's College, Midnapore, Dist: Paschim Medinipur, Pin: 721102, West Bengal, India.
Saudi J Kidney Dis Transpl. 2010 Jan;21(1):75-80.
Dehydration or water deprivation in the body decreases urinary excretion and allows urea and other protein waste products to accumulate in the blood. The aim of the present study is to evaluate the association of uremia and oxidative stress by applying the herbal plant Withania somnifera (W. somnifera) (Aswagandha). The study was performed on male Wister strain rats in which, dehydration was achieved by water withdrawal. A total of 18 rats were studied and were randomly divided into three Groups: Group-1, control, Group-2, only dehydration and Group-3, dehydration + administration of aqueous root extract of W. somnifera, orally (50 mg/100 gm body weight/day) for 25 days. After 25 days of treatment, it was observed that the body weight of Group-3 animals had increased significantly, while that in Group-2 had decreased significantly. The liver enzymes in both blood and kidneys did not show any significant change in the three groups implying absence of any toxicity of the root extract. In Group-2 animals, the serum urea and creatinine levels increased sig-nificantly when compared with animals in Groups-1 and 3. The low levels of serum urea and crea-tinine in Group-3 animals indicates the protective effect of the plant extract against renal injury caused by dehydration. Dehydration-induced oxidative stress was established in our study by noting the low activities of super-oxide dismutase and catalase, both important antioxidant enzymes, in Group-2 animals; both enzymes were stabilized in animals of Groups-3 and 1. In conclusion, it is hypothesized that there is an antioxidative role of W. somnifera resulting in reducing the extent of renal injury as a result of oxidative stress.
体内脱水或水分缺失会减少尿液排泄，使尿素及其他蛋白质代谢废物在血液中蓄积。本研究旨在通过应用草本植物睡茄（Withania somnifera）（印度人参）来评估尿毒症与氧化应激之间的关联。该研究以雄性Wister品系大鼠为对象，通过禁水实现脱水。总共对18只大鼠进行了研究，并将其随机分为三组：第一组为对照组；第二组仅进行脱水处理；第三组进行脱水处理并口服睡茄根水提取物（50毫克/100克体重/天），持续25天。治疗25天后，观察到第三组动物的体重显著增加，而第二组动物的体重显著下降。三组动物血液和肾脏中的肝酶均未显示出任何显著变化，这表明根提取物没有任何毒性。与第一组和第三组动物相比，第二组动物的血清尿素和肌酐水平显著升高。第三组动物血清尿素和肌酐水平较低，表明该植物提取物对脱水引起的肾损伤具有保护作用。在我们的研究中，通过观察到第二组动物中超氧化物歧化酶和过氧化氢酶（两种重要的抗氧化酶）的活性较低，证实了脱水诱导的氧化应激；第三组和第一组动物中的这两种酶活性均稳定。总之，据推测睡茄具有抗氧化作用，可减轻氧化应激导致的肾损伤程度。
