Somily Ali M, Al-Khattaf Abdulaziz S, Kambal Abdelmageed M
Department of Pathology/Microbiology, College of Medicine & King Saud University, King Khalid University Hospital, Riyadh, Kingdom of Saudi Arabia.
Saudi Med J. 2010 Jan;31(1):18-24.
To test the activity of tigecycline against bacterial isolates including multi-drug resistant (MDR) gram negative and gram positive organisms from intensive care patients.
Clinically significant gram positive and MDR gram negative isolates from specimens of patients in the intensive care units of King Khalid University Hospital (KKUH), Riyadh, Kingdom of Saudi Arbia between November 1, 2006 and December 31, 2008 were tested against tigecycline by disc diffusion (DD) method. In some isolates, the minimal inhibitory concentration was carried out by E-test method. Some of the gram negative isolates, and gram positive isolates were tested using both methods. The study was approved by the hospital ethics committee.
All the 83 gram positive organisms tested by both DD and E-test were susceptible to tigecycline. Two hundred and fifty-four MDR gram negative isolates were tested for susceptibility to tigecycline. Of these 176 tested by DD, 159 (90%) were susceptible, 6 (3.4%) were resistant, and 11 (6.2%) were intermediately susceptible (data are not the same in table 3). From the 188 isolates tested by E-test, 140 (74.4%) were susceptible, 35 (18.6%) were resistant, and 13 (6.9%) showed intermediate susceptibility. For comparison between the methods, 109 isolates of the MDR gram negative organisms were tested by both E test and DD. The difference between the 2 methods was not significant.
Tigecycline was active against gram positive and most MDR gram negative isolates from patients in medical and surgical intensive cases in KKUH. There was no significant difference between the DD and E-test methods for susceptibility testing of tigecycline against these isolates.
检测替加环素对包括来自重症监护患者的多重耐药（MDR）革兰氏阴性和革兰氏阳性菌分离株的活性。
对2006年11月1日至2008年12月31日期间沙特阿拉伯利雅得哈立德国王大学医院（KKUH）重症监护病房患者标本中具有临床意义的革兰氏阳性和MDR革兰氏阴性分离株，采用纸片扩散法（DD）检测其对替加环素的敏感性。在一些分离株中，采用E-test法测定最低抑菌浓度。部分革兰氏阴性分离株和革兰氏阳性分离株同时采用这两种方法进行检测。该研究获得了医院伦理委员会的批准。
通过DD法和E-test法检测的所有83株革兰氏阳性菌均对替加环素敏感。对254株MDR革兰氏阴性分离株进行了替加环素敏感性检测。其中，176株采用DD法检测，159株（90%）敏感，6株（3.4%）耐药，11株（6.2%）中介敏感（表3中的数据不同）。采用E-test法检测的188株分离株中，140株（74.4%）敏感，35株（18.6%）耐药，13株（6.9%）中介敏感。为比较两种方法，对109株MDR革兰氏阴性菌同时采用E-test法和DD法进行检测。两种方法之间的差异无统计学意义。
替加环素对KKUH内科和外科重症患者中的革兰氏阳性菌和大多数MDR革兰氏阴性分离株具有活性。DD法和E-test法在检测替加环素对这些分离株的敏感性方面无显著差异。
