光辅助合成用于血清中甲状腺球蛋白检测的发光甘露糖-Au 纳米点。

Photoassisted synthesis of luminescent mannose-Au nanodots for the detection of thyroglobulin in serum.

机构信息

Institute of Bioscience and Biotechnology, Center for Marine Bioscience and Biotechnology, National Taiwan Ocean University, 2, Beining Road, Keelung, 20224, Taiwan.

出版信息

Chem Asian J. 2010 Feb 1;5(2):334-41. doi: 10.1002/asia.200900346.

DOI:10.1002/asia.200900346

PMID:20063339

Abstract

We have employed mannose-modified gold nanodots (Man-Au NDs) as a luminescence sensor for the detection of the thyroid-cancer marker thyroglobulin (Tg) in homogeneous solutions. The luminescent Man-Au NDs are prepared through the reaction of 2.9 nm-diameter gold nanoparticles (Au NPs) with 11-mercapto-3,6,9-trioxaundecyl-alpha-D-mannopyranoside (Man-RSH) under the irradiation of a light-emitting diode (LED). We have found that the irradiation enhances the quantum yield (approximately 11%), alters the emission wavelength and lifetimes, and shortens the preparation time. A luminescence assay has been developed for Tg based on the competition between Tg and Man-Au NDs for the interaction with the concanavalin A (Con A). Because luminescence quenching of the Man-Au NDs by Con A is inhibited by Tg selectivity, we have obtained a highly sensitive and selective assay for Tg.

摘要

我们采用甘露糖修饰的金纳米点（Man-Au NDs）作为荧光传感器，用于在均相溶液中检测甲状腺癌标志物甲状腺球蛋白（Tg）。通过在发光二极管（LED）照射下，将 2.9nm 直径的金纳米粒子（Au NPs）与 11-巯基-3,6,9-三氧杂十一烷基-α-D-甘露吡喃糖苷（Man-RSH）反应，制备出具有荧光的 Man-Au NDs。我们发现，辐照增强了量子产率（约 11%），改变了发射波长和寿命，并缩短了制备时间。基于 Tg 与 Man-Au NDs 与伴刀豆球蛋白 A（Con A）相互作用的竞争，我们开发了一种用于 Tg 的荧光测定法。由于 Con A 对 Man-Au NDs 的荧光猝灭被 Tg 选择性抑制，我们获得了一种对 Tg 具有高灵敏度和选择性的测定法。