Institute of Bioscience and Biotechnology, Center for Marine Bioscience and Biotechnology, National Taiwan Ocean University, 2, Beining Road, Keelung, 20224, Taiwan.
Chem Asian J. 2010 Feb 1;5(2):334-41. doi: 10.1002/asia.200900346.
We have employed mannose-modified gold nanodots (Man-Au NDs) as a luminescence sensor for the detection of the thyroid-cancer marker thyroglobulin (Tg) in homogeneous solutions. The luminescent Man-Au NDs are prepared through the reaction of 2.9 nm-diameter gold nanoparticles (Au NPs) with 11-mercapto-3,6,9-trioxaundecyl-alpha-D-mannopyranoside (Man-RSH) under the irradiation of a light-emitting diode (LED). We have found that the irradiation enhances the quantum yield (approximately 11%), alters the emission wavelength and lifetimes, and shortens the preparation time. A luminescence assay has been developed for Tg based on the competition between Tg and Man-Au NDs for the interaction with the concanavalin A (Con A). Because luminescence quenching of the Man-Au NDs by Con A is inhibited by Tg selectivity, we have obtained a highly sensitive and selective assay for Tg.
我们采用甘露糖修饰的金纳米点（Man-Au NDs）作为荧光传感器，用于在均相溶液中检测甲状腺癌标志物甲状腺球蛋白（Tg）。通过在发光二极管（LED）照射下，将 2.9nm 直径的金纳米粒子（Au NPs）与 11-巯基-3,6,9-三氧杂十一烷基-α-D-甘露吡喃糖苷（Man-RSH）反应，制备出具有荧光的 Man-Au NDs。我们发现，辐照增强了量子产率（约 11%），改变了发射波长和寿命，并缩短了制备时间。基于 Tg 与 Man-Au NDs 与伴刀豆球蛋白 A（Con A）相互作用的竞争，我们开发了一种用于 Tg 的荧光测定法。由于 Con A 对 Man-Au NDs 的荧光猝灭被 Tg 选择性抑制，我们获得了一种对 Tg 具有高灵敏度和选择性的测定法。
