Kurskaia O G, Durymanov A G, Sharshov K A, Zaĭkovskaia A V, Shestopalov A M, Il'icheva T N
Zh Mikrobiol Epidemiol Immunobiol. 2009 Sep-Oct(5):75-9.
To compare usage of native and formalinized erythrocytes from different animal species in hemagglutination inhibition (HI) test for detection of level of specific antibodies to H5N1 influenza virus in sera of mammals.
Level of anti-H5 antibodies to influenza H5 control antigen and to influenza viruses A/Common gull/Chany/2006 (H5N1), A/duck/Tuva/01/06 (H5N1), A/Anas platyrhynchos/Chany Lake/9/03 (H5N3) was determined by hemagglutination inhibition test in two influenza A (H5) reference antisera as well as in ferret antisera to native strains of avian influenza virus. Equine, rhesus macaque, sheep, guinea pig, goose, and chicken erythrocytes were used.
Using reference antisera, H5 hemagglutinin was detected in all tested antigens with all used erythrocytes. While testing ferret antisera in HI test with reference antigen, anti-H5 antibodies were not detected or detected in extremely low titre (1/80) and only with equine erythrocytes. In most cases, titers of anti-H5 antibodies in HI test with formalinized erythrocytes were higher than with native ones.
During monitoring for antibodies to H5N1 avian influenza virus in human population it is necessary to use native strains of A/H5N1 along with reference antigen. It is possible to use formalinized equine, rhesus macaque, goose, and chicken erythrocytes.
比较不同动物来源的天然红细胞和甲醛固定红细胞在血凝抑制（HI）试验中用于检测哺乳动物血清中H5N1流感病毒特异性抗体水平的情况。
采用血凝抑制试验，在两种甲型（H5）参考抗血清以及雪貂针对禽流感病毒天然毒株的抗血清中，测定针对流感H5对照抗原以及甲型流感病毒A/普通海鸥/恰尼/2006（H5N1）、A/鸭/图瓦/01/06（H5N1）、A/绿头鸭/恰尼湖/9/03（H5N3）的抗H5抗体水平。使用了马、恒河猴、绵羊、豚鼠、鹅和鸡的红细胞。
使用参考抗血清时，所有使用的红细胞在所有测试抗原中均检测到H5血凝素。在用参考抗原进行HI试验检测雪貂抗血清时，未检测到抗H5抗体，或仅在极低滴度（1/80）下检测到，且仅使用马红细胞时检测到。在大多数情况下，用甲醛固定红细胞进行HI试验时抗H5抗体的滴度高于使用天然红细胞时。
在监测人群中H5N1禽流感病毒抗体时，有必要同时使用A/H5N1天然毒株和参考抗原。可以使用甲醛固定的马、恒河猴、鹅和鸡的红细胞。
