Bioprospecting and Molecular Pharmacology Laboratory, Division of Molecular Medicine and Cancer Biology, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala 695014, India.
J Ethnopharmacol. 2010 Mar 2;128(1):166-71. doi: 10.1016/j.jep.2010.01.002. Epub 2010 Jan 11.
Cheilanthes farinosa (Forsk.) Kaulf., family: Adianthaceae, is a fern of immense medicinal properties used in ethno-medicine. The Gaddis tribe of Himachal Pradesh, India, has been using this fern to treat liver damage. Aim of the current study was to determine the apoptosis inducing and cytotoxic activity, if any, of this fern towards hepatic cancer cells.
Water extract of the plant was used in the study. MTT assay was performed in hepatocellular carcinoma cell line, Hep3B as well as murine macrophage cell line, RAW264.7 to analyze the cytotoxic activity of the plant. Further, the apoptosis inducing action of water extract of the plant was evaluated using comet assay, DNA fragmentation analysis, DAPI staining of chromatin and Annexin V-FITC staining.
This plant was found to produce considerable cytotoxicity in hepatoma cell line, Hep3B without inducing substantial damage to non-cancerous cell line RAW264.7. In addition, this plant was found to induce apoptosis in Hep3B cells. This was substantiated by comet assay, DNA fragmentation analysis, DAPI staining of chromatin and Annexin V-FITC staining for detecting early stage of apoptosis.
This investigation shows that the water extract of Cheilanthes farinosa has antiproliferative and apoptotic activity in human liver cancer cells and is not deleterious towards non-cancerous macrophage cell line.
Cheilanthes farinosa（Forsk.）Kaulf.，隶属于凤尾蕨科，是一种具有巨大药用价值的蕨类植物，在民族医学中被广泛应用。印度喜马偕尔邦的加迪部落一直用这种蕨类植物来治疗肝损伤。本研究旨在确定该蕨类植物对肝癌细胞是否具有诱导细胞凋亡和细胞毒性作用。
本研究采用该植物的水提物。采用 MTT 法在人肝癌细胞系 Hep3B 及小鼠巨噬细胞系 RAW264.7 中检测植物的细胞毒性。进一步采用彗星试验、DNA 片段化分析、染色质 DAPI 染色和 Annexin V-FITC 染色评估植物水提物的诱导细胞凋亡作用。
该植物对肝癌细胞系 Hep3B 产生了显著的细胞毒性作用，而对非癌细胞系 RAW264.7 没有造成实质性损伤。此外，该植物被发现能诱导 Hep3B 细胞凋亡。彗星试验、DNA 片段化分析、染色质 DAPI 染色和 Annexin V-FITC 染色均证实了这一点，这些方法用于检测早期凋亡。
本研究表明，凤尾蕨的水提物对人肝癌细胞具有抗增殖和诱导凋亡作用，且对非癌细胞系巨噬细胞没有损伤作用。
