Andrade Juliana Mm, Passos Carolina Dos S, Dresch Roger R, Kieling-Rubio Maria Angélica, Moreno Paulo Roberto H, Henriques Amélia T
Faculty of Pharmacy, Federal University of Rio Grande do Sul (UFRGS), Av. Ipiranga 2752, 90.610-000, Porto Alegre, RS, Brazil.
Department of Botany, UFRGS, Av. Bento Gonçalves, 9500, 91.501-970, Porto Alegre, RS, Brazil.
Pharmacogn Mag. 2014 Jan;10(Suppl 1):S100-9. doi: 10.4103/0973-1296.127354.
Ferns are a group of plants that have been little explored from a chemical and biological perspective but that have interesting potential, occurring in various parts of the world.
This work investigates the chemical profile and the biological effects of ferns from Brazil.
Analyses were performed using rapid performance liquid chromatography (RP-LC) with a diode array detector (DAD). Extracts were tested for their in vitro antioxidant activity, by the total reactive antioxidant potential method and for their antichemotactic potential, by the Boyden chamber method. Cytotoxic effects were assessed by lactate dehydrogenase levels, while the monoamine oxidase (MAO) assay was carried out using a fluorescence-based method.
Different chemical compositions were found for the studied ferns, such as Asplenium gastonis, in which hesperidin was identified in its extract, while A. serra showed the presence of xanthone mangiferin. The most samples with highest antioxidant activity were the Asplenium serra, Lastreopsis amplissima and Cyathea dichromatolepis extracts, at 10 μg/mL. High antichemotactic activity was found for A. serra (94.06%) and Didymochlaena truncatula (93.41%), at 10 μg/mL. The extracts showed no cytotoxicity at the highest concentration. Against MAO-A, D. truncatula (82.61%), Alsophila setosa (82.21%), Cyathea phalerata (74.07%) and C. delgadii (70.32%) were the most active extracts (100 μg/mL).
The hypothesis was considered that phenolics and triterpenes are responsible for these pronounced activities.
蕨类植物是一类从化学和生物学角度研究较少但具有有趣潜力的植物，分布于世界各地。
本研究调查巴西蕨类植物的化学特征和生物学效应。
采用带二极管阵列检测器（DAD）的快速高效液相色谱（RP-LC）进行分析。通过总反应抗氧化能力法检测提取物的体外抗氧化活性，采用Boyden小室法检测其抗趋化活性。通过乳酸脱氢酶水平评估细胞毒性，同时采用基于荧光的方法进行单胺氧化酶（MAO）检测。
在所研究的蕨类植物中发现了不同的化学成分，如在加斯通铁角蕨提取物中鉴定出橙皮苷，而锯叶铁角蕨中存在呫吨酮芒果苷。抗氧化活性最高的样品大多是10μg/mL浓度下的锯叶铁角蕨、大叶黑桫椤和双色桫椤提取物。在10μg/mL浓度下，锯叶铁角蕨（94.06%）和截叶黑桫椤（93.41%）具有较高的抗趋化活性。提取物在最高浓度下未显示细胞毒性。在100μg/mL浓度下，截叶黑桫椤（82.61%）、毛轴桫椤（82.21%）、鳞盖桫椤（74.07%）和德尔加迪桫椤（70.32%）对MAO-A的抑制活性最高。
酚类和三萜类物质被认为是这些显著活性的原因。
