Institute of Pharmaceutical Sciences, Pharmacognosy, Karl-Franzens-University Graz, Universitätsplatz 4, 8010 Graz, Austria.
J Ethnopharmacol. 2010 Mar 2;128(1):184-97. doi: 10.1016/j.jep.2010.01.007. Epub 2010 Jan 11.
For the assessment of the in vitro anti-protozoal potential of plants traditionally used in Ecuador in the treatment of leishmaniasis, a combined approach based on interviews with healers as well as a literature search was carried out.
From three regions of Ecuador, 256 local healers called "Agents of Traditional Medicine" (ATMs) were interviewed about their knowledge of the use of plants to treat and heal the illness recognized by the ATMs as leishmaniasis. From literature sources, 14 plants were identified as being used in the treatment of leishmaniasis. Subsequently, plant material was collected from a representative selection of 39 species. A total of 140 extracts were screened in vitro against Leishmania donovani, Plasmodium falciparum, Trypanosoma brucei rhodesiense and Trypanosoma cruzi. Additionally, these extracts were evaluated for their anti-microbial activities using five gram-positive and -negative bacteria as well as Candida albicans.
The survey resulted in 431 use-records for 145 plant-taxa used for the treatment of leishmaniasis. The 10 most frequently reported taxa accounted for 37.7% of all records. In the case of leishmaniasis, activity was observed for Elephantopus mollis, Minquartia guianensis, Bocconia integrifolia, Gouania lupuloides, Scoparia dulcis, an as-yet-unidentified species of Piper and Brugmansia. For the leaves of M. guianensis and the twigs and bark of G. lupuloides a good selectivity index (SI) was found. IC(50) values and the SI of active plant extracts are presented.
为了评估在厄瓜多尔传统用于治疗利什曼病的植物的体外抗原生动物潜力，我们采用了一种结合采访治疗师和文献检索的方法。
我们采访了来自厄瓜多尔三个地区的 256 名被称为“传统医学代理人”（ATMs）的当地治疗师，了解他们使用植物治疗和治愈 ATMs 所认可的利什曼病的知识。从文献来源中，确定了 14 种植物用于治疗利什曼病。随后，从具有代表性的 39 种植物中收集了植物材料。总共对 140 种提取物进行了体外筛选，以对抗利什曼原虫、恶性疟原虫、布氏锥虫和克氏锥虫。此外，还使用五种革兰氏阳性和阴性细菌以及白色念珠菌评估了这些提取物的抗微生物活性。
调查结果导致了 431 项用于治疗利什曼病的 145 种植物分类群的使用记录。报告最多的 10 种分类群占所有记录的 37.7%。在利什曼病的情况下，观察到 Elephantopus mollis、Minquartia guianensis、Bocconia integrifolia、Gouania lupuloides、Scoparia dulcis、一种尚未鉴定的 Piper 种和 Brugmansia 具有活性。对于 M. guianensis 的叶子和 G. lupuloides 的嫩枝和树皮，发现了良好的选择性指数（SI）。本文提供了 IC50 值和活性植物提取物的 SI。
