Faculty of Pharmaceutical Sciences, University of Kinshasa, PO. Box 212, Kinshasa XI, Congo.
J Ethnopharmacol. 2012 May 7;141(1):301-8. doi: 10.1016/j.jep.2012.02.035. Epub 2012 Mar 3.
The antiprotozoal and cytotoxic activity of the aqueous extracts from 33 medicinal plants, used by traditional healers for the treatment of various parasitic diseases and collected after an ethnopharmacological inventory conducted in the Bolongo area, Bandundu province in DR Congo, was evaluated.
Decoctions were prepared, lyophilized and evaluated for in vitro antiprotozoal activity against Trypanosoma b. brucei, Trypanosoma cruzi, Leishmania infantum, and the chloroquine- and pyrimethamine-resistant K1 strain of Plasmodium falciparum. Cytotoxicity against MRC-5 cells was included to assess selectivity of activity.
Most of the tested extracts exhibited pronounced (IC(50)≤5μg/ml) or good (5<IC(50)≤10μg/ml) antiprotozoal activity against one or more of the selected protozoa. A total of 19 plant extracts inhibited Trypanosoma b. brucei, especially the extract from Isolona hexaloba stem bark (IC(50)=1.95μg/ml, SI=16.5); 8 plant extracts were active against Trypanosoma cruzi, the extracts from Enanatia chlorantha stem bark and Quassia africana root bark being the most active with IC(50) values of 1.87 and 1.88μg/ml, respectively (SI=3.0 and 3.3, respectively); 8 plant extracts showed activity against Leishmania infantum, with extracts from Napoleona vogelii stem bark and Quassia africana root bark as the most active with IC(50) values of 5.66 and 5.04μg/ml (SI=11.3 and 1.2). Finally, 9 plant extracts inhibited Plasmodium falciparum K1 with the extracts from Quassia africana (root bark and stem bark) being the most active ones with IC(50) values of 0.46 and 1.27μg/ml (SI=13.7 and 13.6). Extracts from Enantia chlorantha stem bark, Piptadeniastrum africanum stem bark and Quassia africana root bark were cytotoxic for MRC-5 cells (CC(50)<10μg/ml).
These results can partly support and justify the traditional use of some of these plant species for the treatment of parasitic diseases.
评估了来自 33 种药用植物的水提取物的抗原生动物和细胞毒性活性，这些植物是传统治疗师用于治疗各种寄生虫病的，并且是在刚果民主共和国邦多省的博隆戈地区进行民族药理学调查后收集的。
制备煎剂，冻干并评估其对 Trypanosoma b. brucei、Trypanosoma cruzi、Leishmania infantum 和氯喹和乙胺嘧啶耐药的 Plasmodium falciparum K1 株的体外抗原生动物活性。包括对 MRC-5 细胞的细胞毒性作用以评估活性的选择性。
大多数测试提取物对所选原生动物中的一种或多种表现出明显的（IC50≤5μg/ml）或良好的（5<IC50≤10μg/ml）抗原生动物活性。共有 19 种植物提取物抑制了 Trypanosoma b. brucei，特别是从 Isolona hexaloba 树皮中提取的提取物（IC50=1.95μg/ml，SI=16.5）；8 种植物提取物对 Trypanosoma cruzi 具有活性，其中 Enanatia chlorantha 树皮和 Quassia africana 根皮提取物的活性最强，IC50 值分别为 1.87 和 1.88μg/ml（SI 分别为 3.0 和 3.3）；8 种植物提取物对 Leishmania infantum 具有活性，其中 Napoleona vogelii 树皮和 Quassia africana 根皮提取物的活性最强，IC50 值分别为 5.66 和 5.04μg/ml（SI 分别为 11.3 和 1.2）。最后，9 种植物提取物抑制 Plasmodium falciparum K1，其中 Quassia africana（根皮和树皮）提取物的活性最强，IC50 值分别为 0.46 和 1.27μg/ml（SI 分别为 13.7 和 13.6）。Enantia chlorantha 树皮、Piptadeniastrum africanum 树皮和 Quassia africana 根皮提取物对 MRC-5 细胞具有细胞毒性（CC50<10μg/ml）。
这些结果可以部分支持和证明传统上使用这些植物物种治疗寄生虫病的合理性。
