Ar'Rajab A, Bengmark S, Ahrén B
Department of Surgery, Lund University, Sweden.
Transplantation. 1991 Mar;51(3):570-4. doi: 10.1097/00007890-199103000-00005.
Islet transplantation may be optimized by islet immunoisolation to prevent direct contact between the islet graft and the host tissue. In this study, we examined the glycemia and insulin secretion in streptozotocin-diabetic rats transplanted with islets subjected to immunoisolation with Algire diffusion chamber or with microencapsulation. Two days after diabetes induction by streptozotocin (70 mg/kg i.v.), rats were transplanted i.p. with either 1500 or 3000 islets encapsulated in Algire diffusion chambers, or with either 1500 or 3000 microencapsulated islets. Controls were diabetic rats transplanted i.p. with 1500 overnight-cultured islets not subjected to immunoisolation. In these controls, normoglycemia was evident for 3 weeks and a normal plasma insulin response to glucose infusion (10 mg/min) was seen at day 10 after transplantation. It was found that rats transplanted with 1500 microencapsulated islets similarly were normoglycemic for 3 weeks and that the plasma insulin response to glucose infusion at day 10 was normal. Furthermore, rats transplanted with 3000 microencapsulated islets remained normoglycemic for 6 months, and a glucose infusion performed at 6 months in these rats showed a normal acute plasma insulin response, whereas the second phase of insulin secretion was reduced. In contrast, rats transplanted with 1500 islets immunoisolated in Algire chamber remained hyperglycemic, and rats transplanted with 3000 islets within Algire chamber were normoglycemic for only 2 weeks. We conclude that microencapsulation is superior to the use of diffusion chamber as the immunoisolation technique for islets used for transplantation.
胰岛移植可通过胰岛免疫隔离进行优化，以防止胰岛移植物与宿主组织直接接触。在本研究中，我们检测了接受阿尔吉尔扩散室免疫隔离或微囊化处理的胰岛移植到链脲佐菌素诱导的糖尿病大鼠后的血糖水平和胰岛素分泌情况。用链脲佐菌素（70mg/kg静脉注射）诱导糖尿病两天后，大鼠经腹腔注射移植1500个或3000个封装在阿尔吉尔扩散室内的胰岛，或1500个或3000个微囊化胰岛。对照组为经腹腔注射移植1500个未进行免疫隔离的过夜培养胰岛的糖尿病大鼠。在这些对照组中，移植后3周血糖正常，移植后第10天可见血浆胰岛素对葡萄糖输注（10mg/min）的正常反应。结果发现，移植1500个微囊化胰岛的大鼠同样在3周内血糖正常，且移植后第10天血浆胰岛素对葡萄糖输注的反应正常。此外，移植3000个微囊化胰岛的大鼠在6个月内血糖一直正常，在6个月时对这些大鼠进行葡萄糖输注显示急性血浆胰岛素反应正常，而胰岛素分泌的第二阶段有所降低。相比之下，移植1500个在阿尔吉尔扩散室内免疫隔离的胰岛的大鼠仍处于高血糖状态，移植3000个胰岛于阿尔吉尔扩散室内的大鼠仅在2周内血糖正常。我们得出结论，作为用于移植的胰岛的免疫隔离技术，微囊化优于使用扩散室。
