Robley Rex Department of Veterans Affairs Medical Center, Louisville, KY, USA.
Islets. 2011 May-Jun;3(3):89-92. doi: 10.4161/isl.3.3.15404. Epub 2011 May 1.
The progress of immunoisolation as a treatment for diabetes has been hampered by the diminished long term viability of islets within the immunoisolation device. Chronic hypoxia is greatly responsible for islet cell death within an immunoisolation device and remains an obstacle to the success of this form of islet transplantation. In order to address this problem, isolated rat islets were transfected with a plasmid encoding cytoglobin, an intracellular oxygen binding protein. Untreated or transfected islets were placed in polyacrylonitrile-polyvinychloride hollow fiber and implanted beneath the hepatic capsule in streptozotocin-diabetic rats. Fasting blood glucose was used as an indicator of islet survival and function. Rats receiving fibers containing transfected islets remained normoglycemic through the 60 day trial. Untreated islets failed within two weeks after implantation resulting in elevated blood glucose in the recipient. The fibers were recovered and tested for insulin content. Cytoglobin promoted islet cell survival and insulin synthesis and secretion. The induction of cytoglobin in islets may reduce cell loss from chronic hypoxia and may be a useful method to improve the feasibility of immunoisolation as an islet transplantation modality.
免疫隔离作为治疗糖尿病的一种方法，其进展一直受到隔离装置内胰岛长期存活率降低的阻碍。慢性缺氧是免疫隔离装置中胰岛细胞死亡的主要原因，也是这种胰岛移植形式成功的障碍。为了解决这个问题，将编码细胞结合珠蛋白（一种细胞内氧结合蛋白）的质粒转染到分离的大鼠胰岛中。未处理或转染的胰岛被放置在聚丙烯腈-聚氯乙烯中空纤维中，并植入链脲佐菌素糖尿病大鼠的肝包膜下。空腹血糖被用作胰岛存活和功能的指标。接受含有转染胰岛的纤维的大鼠在 60 天的试验中保持正常血糖水平。未处理的胰岛在植入后两周内失效，导致受体的血糖升高。回收纤维并测试胰岛素含量。细胞结合珠蛋白促进胰岛细胞存活和胰岛素的合成和分泌。在胰岛中诱导细胞结合珠蛋白可能会减少慢性缺氧引起的细胞丢失，并且可能是改善免疫隔离作为胰岛移植方式可行性的有用方法。
