Krestow M, Lum Z P, Tai I T, Sun A
Department of Physiology, University of Toronto, Ontario, Canada.
Transplantation. 1991 Mar;51(3):651-5. doi: 10.1097/00007890-199103000-00021.
Fetal pancreatic islets were isolated from 21-day pregnant Wistar rats and enclosed in semipermeable alginate-polylysine-alginate capsules. Encapsulated islets that had been previously cultured for eight days in vitro were shown to secrete insulin in response to glucose challenge: low-glucose, high-glucose, and high-glucose + 3-isobutyl-1-methyl-xanthine (IBMX). Transplants of 800-1000 encapsulated cultured fetal islets into the peritoneal cavities of BALB/c mice with streptozotocin-induced diabetes restored normoglycemia for up to 171 days without immunosuppression. When the capsules were removed from 2 of the recipients they both quickly regressed to a diabetic state. Control groups of diabetic mice received unencapsulated, uncultured islets or empty capsules. The mortality rate among these animals was high and none experienced relief from hyperglycemia for longer than 6 days. These results demonstrate that cultured microencapsulated fetal rat islets of Langerhans can release insulin in response to an in vitro glucose challenge, and that transplants of these islets into diabetic mice can restore normoglycemia without the need for immunosuppressive therapy.
从怀孕21天的Wistar大鼠中分离出胎儿胰岛，并将其包裹在半透性藻酸盐-聚赖氨酸-藻酸盐胶囊中。先前在体外培养8天的包封胰岛显示，在葡萄糖刺激下会分泌胰岛素：低葡萄糖、高葡萄糖以及高葡萄糖 + 3-异丁基-1-甲基黄嘌呤（IBMX）。将800 - 1000个包封的培养胎儿胰岛移植到链脲佐菌素诱导的糖尿病BALB/c小鼠的腹腔中，在不进行免疫抑制的情况下，血糖正常状态可维持长达171天。当从2只受体小鼠中取出胶囊时，它们都迅速恢复到糖尿病状态。糖尿病小鼠的对照组接受未包封、未培养的胰岛或空胶囊。这些动物的死亡率很高，且没有一只动物的高血糖缓解超过6天。这些结果表明，培养的微囊化胎儿大鼠胰岛可对体外葡萄糖刺激作出反应释放胰岛素，并且将这些胰岛移植到糖尿病小鼠中可恢复血糖正常状态，而无需免疫抑制治疗。
