Department of Pathophysiology, Jagiellonian University, Medical College, Cracow, Poland.
J Physiol Pharmacol. 2009 Dec;60(4):85-91.
Previous studies have suggested that, different types of unmyelinated bladder afferent C-fibres, such as capsaicin-sensitive and capsaicin-resistant mediate the voiding reflex in overactive bladder (OAB). Considering its polymodal features, we explored the urodynamic effect of primary afferent neurons modulation on detrusor activity in normal and OAB rats. Experiments were performed on 48 female rats. OAB was induced by intraperitoneal administration of cyclophosphamide. All the surgical procedures and urodynamic studies were performed under urethane anaesthesia. Cystometry was done after a 1 h recovery period from the surgical procedure. All animals were randomly divided into six groups: control, chronic OAB, chronic OAB after capsaicin or lidocaine instillation, control capsaicin or lidocaine instillation. The measurements represent the average of five bladder micturition cycles. We analyzed: basal, threshold, micturition voiding pressure; intercontraction interval; compliance; functional bladder capacity; motility index; detrusor overactivity index. We used chronic cyclophosphamide OAB model for further investigations. In healthy rats, intravesical instillation of capsaicin caused complete inhibition of detrusor contractility preventing from proper voiding function of the bladder. Contrary, lidocaine has no influence on micturition cycles in intact animals. Also, intravesical instillation of capsaicin and lidocaine reduced the severity of detrusor overactivity of OAB rats leading to improvement of cystometric parameters.
先前的研究表明，不同类型的无髓鞘膀胱传入 C 纤维，如辣椒素敏感和辣椒素抗性纤维，介导逼尿反射过度活跃（OAB）。考虑到其多模态特征，我们探索了初级传入神经元调节对正常和 OAB 大鼠膀胱活动的尿动力学影响。实验在 48 只雌性大鼠上进行。通过腹腔内给予环磷酰胺诱导 OAB。所有手术程序和尿动力学研究均在乌拉坦麻醉下进行。手术后 1 小时恢复期进行膀胱测压。所有动物随机分为六组：对照组、慢性 OAB 组、慢性 OAB 后辣椒素或利多卡因灌注组、对照组辣椒素或利多卡因灌注组。测量值代表五个膀胱排尿周期的平均值。我们分析了：基础、阈值、排尿排空压力；收缩间期；顺应性；功能性膀胱容量；运动指数；逼尿肌过度活动指数。我们使用慢性环磷酰胺 OAB 模型进行进一步研究。在健康大鼠中，膀胱内灌注辣椒素可完全抑制逼尿肌收缩性，从而防止膀胱正常排空功能。相反，利多卡因对完整动物的排尿周期没有影响。此外，膀胱内灌注辣椒素和利多卡因可减轻 OAB 大鼠逼尿肌过度活动的严重程度，从而改善膀胱测压参数。
