希腊中部血液透析患者隐匿性乙型肝炎病毒感染的流行情况。
Prevalence of occult hepatitis B virus infection in haemodialysis patients from central Greece.
机构信息
Department of Medicine and Research Laboratory of Internal Medicine, Medical School, University of Thessaly, 41110 Larissa, Thessaly, Greece.
出版信息
World J Gastroenterol. 2010 Jan 14;16(2):225-31. doi: 10.3748/wjg.v16.i2.225.
AIM
To assess the hepatitis B virus (HBV)-DNA and the prevalence of occult HBV infection in end-stage renal failure (ESRF) patients from Central Greece.
METHODS
Sera from 366 ESRF patients attending five out of six dialysis units from Central Greece were investigated for HBV-DNA by real-time polymerase chain reaction. Only serum samples with repeatedly detectable HBV-DNA were considered positive. IgG antibodies to hepatitis C virus (anti-HCV) were tested by a third generation enzyme linked immunosorbent assay (ELISA), while IgG antibodies to hepatitis E virus (anti-HEV) were tested by two commercially available ELISAs.
RESULTS
HBV-DNA was detected in 15/366 patients (4.1%) and HBsAg in 20/366 (5.5%). The prevalence of occult HBV infection was 0.9% (3/346 HBsAg-negative patients). Occult HBV was not associated with a specific marker of HBV infection or anti-HCV or anti-HEV reactivity. There was no significant difference in HBV-DNA titres, demographic and biochemical features, between patients with occult HBV infection and those with HBsAg-positive chronic HBV infection.
CONCLUSION
In central Greece, 4% of ESRF patients had detectable HBV-DNA, though in this setting, the prevalence of occult HBV seems to be very low (0.9%).
目的
评估来自希腊中部的终末期肾衰竭（ESRF）患者乙型肝炎病毒（HBV）-DNA 与隐匿性 HBV 感染的流行情况。
方法
对来自希腊中部的六个透析中心中的五个中心的 366 例 ESRF 患者的血清进行了 HBV-DNA 的实时聚合酶链反应检测。只有可重复检测到 HBV-DNA 的血清样本才被认为是阳性。丙型肝炎病毒（抗-HCV）的 IgG 抗体采用第三代酶联免疫吸附试验（ELISA）进行检测，而戊型肝炎病毒（抗-HEV）的 IgG 抗体则采用两种市售的 ELISA 进行检测。
结果
在 366 例患者中，有 15 例（4.1%）检测到 HBV-DNA，20 例（5.5%）检测到 HBsAg。隐匿性 HBV 感染的流行率为 0.9%（346 例 HBsAg 阴性患者中的 3 例）。隐匿性 HBV 与 HBV 感染的特定标志物、抗-HCV 或抗-HEV 反应无关。在隐匿性 HBV 感染患者和 HBsAg 阳性慢性 HBV 感染患者之间，HBV-DNA 滴度、人口统计学和生化特征均无显著差异。
结论
在希腊中部，4%的 ESRF 患者可检测到 HBV-DNA，但在这种情况下，隐匿性 HBV 的流行率似乎非常低（0.9%）。
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