Zhang Chunxia, Yang Lixin, Yu Xing, Yu Shengxian, Yuan Ya'nan, Chen Chengyu, Yang Bin, Liao Chaolin, You Jinwen
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2009 Oct;34(19):2431-7.
The effects of tree ages and geographic area on quality of bark of Magnolia officinalis (MO) and M. officinalis var. biloba (MOB) were studied. The volatile oils of samples extracted by a water stream distillation method were analyzed using a GC/MS method. The non-volatile ingredients of samples were determined by an HPLC method. Seven and twenty characteristic peaks were detected in the HPLC and GC fingerprint of MO, respectively, and the similar HPLC and GC fingerprint were showed in MOB. For samples collected in the same geographic area with different ages, the concentrations of active ingredients varied barely, whereas for those samples collected from different geographic area, differences on the concentrations of active ingredients were observed, for example, the concentrations of the amount of magnolol and honokiol in samples collected in Hubei province were higher than those collected in other areas. Big differences on the concentrations of active ingredients were found between MO and MOB, for MO, the ratio of honokiol/ magnolol was more than 0.7, whereas the ratio-was less than 0.7 in the case of MOB, and the concentrations of honokiol in MO were higher than those in MOB.
研究了树龄和地理区域对厚朴（MO）及凹叶厚朴（MOB）树皮质量的影响。采用水蒸气蒸馏法提取样品中的挥发油，并用气相色谱/质谱联用（GC/MS）法进行分析。采用高效液相色谱（HPLC）法测定样品中的非挥发性成分。厚朴的HPLC指纹图谱和GC指纹图谱分别检测到7个和20个特征峰，凹叶厚朴也呈现出相似的HPLC和GC指纹图谱。对于在同一地理区域采集的不同树龄的样品，活性成分的浓度变化不大，而对于从不同地理区域采集的样品，观察到活性成分浓度存在差异，例如，湖北省采集的样品中厚朴酚和和厚朴酚的含量高于其他地区采集的样品。厚朴和凹叶厚朴在活性成分浓度上存在很大差异，对于厚朴，和厚朴酚/厚朴酚的比例大于0.7，而凹叶厚朴的该比例小于0.7，且厚朴中和厚朴酚的浓度高于凹叶厚朴。
