[空心莲子草的成分及其抗肿瘤活性]

[Constituents from Alternanthera philoxeroides and their antitumor activity].

作者信息

Fang Jinbo, Chen Jiachun, Liu Yanwen, Duan Hongquan

机构信息

School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2009 Oct;34(19):2473-6.

PMID:20067016

Abstract

OBJECTIVE

To investigate antitumor constituents from n-butyl alcohol extract of Alternanthera philoxeroides.

METHOD

The constituents were isolated with silica gel, gel permeation chromatography, and purified by HPLC. Their structures were elucidated by spectroscopy and acid hydrolysis. The antitumor effects of extracts and isolated compounds were tested by MTH method in vitro.

RESULT

Seven compounds were isolated and elucidated as followings: oleanolic acid 3-O-beta-D-glucuronopyranoside (1), oleanolic acid 28-O-beta-D-glucopyranoside (2), oleanolic acid 3-O-beta-D-glucuronopyranoside-6'-O-methyl ester (3), chikusetsusaponin IV a methyl ester (4), hederagenin 3-O-beta-D-glucuronopyranoside-6'-O-methyl ester (5), 4,5-dihydroblumenol (6), 6S,7E,9R-6,9-di-hydroxymegastigma-4,7-dien-3-one-9-O-beta-D-glucopyranoside (7). Compound 1 showed significant inhibitory effect against Hela and L929 with inhibitive ratios 91.3% and 92.9% at 30 mg x L(-1), respectively.

CONCLUSION

Compounds 4, 5 and 7 were isolated from this genus for the first time. Compound 1 showed significant inhibitory effect against Hela and L929 at 30 mg x L(-1).

摘要

目的

研究空心莲子草正丁醇提取物中的抗肿瘤成分。

方法

采用硅胶柱色谱、凝胶渗透色谱进行分离，并通过高效液相色谱进行纯化。通过光谱学和酸水解对其结构进行鉴定。采用MTT法体外检测提取物及分离得到的化合物的抗肿瘤作用。

结果

分离并鉴定了7个化合物，分别为齐墩果酸3 - O - β - D - 葡萄糖醛酸吡喃糖苷（1）、齐墩果酸28 - O - β - D - 葡萄糖吡喃糖苷（2）、齐墩果酸3 - O - β - D - 葡萄糖醛酸吡喃糖苷 - 6'- O - 甲酯（3）、竹节人参皂苷IV a甲酯（4）、常春藤皂苷元3 - O - β - D - 葡萄糖醛酸吡喃糖苷 - 6'- O - 甲酯（5）、4,5 - 二氢布鲁门醇（6）、6S,7E,9R - 6,9 - 二羟基大柱头草 - 4,7 - 二烯 - 3 - 酮 - 9 - O - β - D - 葡萄糖吡喃糖苷（7）。化合物1在30 mg·L⁻¹时对Hela细胞和L929细胞显示出显著抑制作用，抑制率分别为91.3%和92.9%。