Lobastova T G, Gulevskaia S A, Sukhodol'skaia G V, Donova M V
Prikl Biokhim Mikrobiol. 2009 Nov-Dec;45(6):684-9.
The ability of 485 fungal strains is studied for catalysis of the process of 7alpha, 15alpha-dihydroxylation of dehydroepiandrosterone (DHEA, 3alpha-hydroxy-5-androstene-17-one), a key intermediate of the synthesis of physiologically active compounds. The ability for the formation of 3alpha, 7alpha, 15alpha-trihydoxy-5-androstene-17-one (7alpha, 15alpha-di-OH-DHEA) was found for the first time for representatives of 12 genera, eight families, and six orders of ascomycetes, eight genera, four families, and one order of zygomycetes, one genus, one family, and one order of basidiomycetes, and four genera of mitosporous fungi. The most active strains are found among genera Acremonium, Gibberella, Fusarium, and Nigrospora. In the process of transformation of DHEA (2 g/l) by strains of Fusarium oxysporum RKM F-1600 and FGibberella zeae BKM F-2600, the molar yield was 63 and 68%, respectively. Application of the revealed active strains of microorganisms opens prospects for the efficient production of key intermediates of synthesis of modern medical preparations.
研究了485株真菌催化脱氢表雄酮（DHEA，3α-羟基-5-雄烯-17-酮）7α,15α-二羟基化过程的能力，DHEA是生理活性化合物合成的关键中间体。首次发现子囊菌纲12个属、8个科和6个目、接合菌纲8个属、4个科和1个目、担子菌纲1个属、1个科和1个目以及4个半知菌类真菌的代表菌株具有形成3α,7α,15α-三羟基-5-雄烯-17-酮（7α,15α-二羟基-DHEA）的能力。最具活性的菌株存在于顶孢霉属、赤霉属、镰刀菌属和黑孢霉属中。在尖孢镰刀菌RKM F-1600和玉米赤霉BKM F-2600菌株转化DHEA（2 g/l）的过程中，摩尔产率分别为63%和68%。所发现的微生物活性菌株的应用为高效生产现代药物制剂合成的关键中间体开辟了前景。
