Department of Rehabilitation, West China Hospital, Sichuan University, Chengdu, China.
Connect Tissue Res. 2010;51(1):1-7. doi: 10.3109/03008200902855917.
This study was designed to determine the effects of pulsed electromagnetic fields (PEMF) on the mRNA expression of the receptor activator of NF-kappa-B (RANK) and carbonic anhydrase II (CA II) in ovariectomized rat osteoclast-like cells. Marrow cells were harvested from femora and tibiae of rats, from which the ovaries had been totally excised, and cultured in 6-well chamber slides. After 1 day of incubation, the marrow cells were exposed to PEMF for 3 days with 3.8 mT, 8 Hz, and 40 min per day. Osteoclast-like cells were confirmed by both tartrate resistant acid phosphatase (TRAP) stain and bone resorption assay. The expression of RANK and CA II mRNA was determined with real-time fluorescent-nested quantitative polymerase chain reaction. Compared with the sham group, the level of serum estradiol in the ovariectomized group was significantly decreased ( p < 0.05). The numbers of multinucleated, TRAP-positive osteoclast-like cells and resorption pits formed were observed. In invitro study, the expression of RANK and CA II were measured in sham, ovariectomized without PEMF, and ovariectomized with PEMF treatment. Compared with the ovariectomized (PEMF) experimental group and sham group, CA II mRNA expression was significantly increased in the ovariectomized control group ( p < 0.05, 0.01, respectively). Compared with the sham group, RANK mRNA expression was significantly increased in the ovariectomized control group ( p < 0.05). These data suggest that PEMF could regulate the expression of RANK and CA II mRNA in the marrow culture system.
本研究旨在探讨脉冲电磁场（PEMF）对去卵巢大鼠破骨细胞样细胞中核因子-κB 受体激活剂（RANK）和碳酸酐酶 II（CA II）mRNA 表达的影响。从大鼠股骨和胫骨中采集骨髓细胞，这些大鼠的卵巢已被全部切除，并在 6 孔室载玻片上培养。孵育 1 天后，将骨髓细胞用 3.8 mT、8 Hz、每天 40 分钟的 PEMF 暴露 3 天。通过抗酒石酸酸性磷酸酶（TRAP）染色和骨吸收试验来确认破骨细胞样细胞。采用实时荧光嵌套定量聚合酶链反应测定 RANK 和 CA II mRNA 的表达。与假手术组相比，去卵巢组血清雌二醇水平明显降低（p <0.05）。观察到多核、TRAP 阳性的破骨细胞样细胞的数量和形成的吸收陷窝。在体外研究中，在假手术、未用 PEMF 处理的去卵巢和用 PEMF 处理的去卵巢组中测量了 RANK 和 CA II 的表达。与去卵巢（PEMF）实验组和假手术组相比，去卵巢对照组 CA II mRNA 表达明显增加（p <0.05，0.01）。与假手术组相比，去卵巢对照组 RANK mRNA 表达明显增加（p <0.05）。这些数据表明，PEMF 可调节骨髓培养系统中 RANK 和 CA II mRNA 的表达。
