Department of Biomedical Engineering, State University of New York at Stony Brook, Stony Brook, NY 11794, USA.
Dig Surg. 2009 Feb;26(6):476-81. doi: 10.1159/000253872. Epub 2010 Jan 8.
BACKGROUND/AIMS: Intra-abdominal adhesions typically occur after surgically damaged tissues are situated in apposition, leading to fibrotic connections. The goal of this study was to demonstrate the in vivo efficacy of a cross-linked and insoluble hyaluronic acid (HA) film to reduce postsurgical adhesion in a rat model.
To measure in vitro adhesion resistance, porcine monocytes were deposited on the surface of films and their attachment was monitored by scanning electron microscopy. A rat cecum abrasion and abdominal insult model was utilized to demonstrate in vivo efficacy. Briefly, an HA film was deployed as a barrier between the damaged cecal and abdominal tissue surfaces for 21 days; control animals did not receive treatment. At the study conclusion, the rats were sacrificed and the degree of adhesion was determined using a scale from 0 to 3, where 0 = no adhesion and 3 = severe fibrosis.
HA films resisted monocyte adhesion in vitro. The in vivo study results demonstrated a significantly lower mean adhesion score (0.625 +/- 0.517) with HA film treatment compared to the controls (2.09 +/- 1.22).
Placement of HA films between injured tissues significantly decreases the severity of abdominal adhesions. Furthermore, the HA film's resistance to monocyte adhesion could be contributory to lowering in vivo adhesion scores.
背景/目的：腹腔粘连通常发生在手术损伤的组织贴合后，导致纤维连接。本研究的目的是在大鼠模型中证明交联和不溶性透明质酸（HA）膜减少术后粘连的体内疗效。
为了测量体外粘连阻力，将猪单核细胞沉积在膜的表面，并通过扫描电子显微镜监测其附着。使用大鼠盲肠磨损和腹部损伤模型来证明体内疗效。简而言之，将 HA 膜作为受损盲肠和腹部组织表面之间的屏障放置 21 天；对照动物未接受治疗。在研究结束时，处死大鼠，使用 0 到 3 的等级来确定粘连程度，其中 0 = 无粘连，3 = 严重纤维化。
HA 膜在体外抵抗单核细胞附着。体内研究结果表明，与对照组（2.09 +/- 1.22）相比，HA 膜治疗的平均粘连评分（0.625 +/- 0.517）显著降低。
在受伤组织之间放置 HA 膜可显著降低腹部粘连的严重程度。此外，HA 膜对单核细胞附着的抵抗力可能有助于降低体内粘连评分。
