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溶藻弧菌中类rpoS σ因子rpoX的克隆、鉴定及特性分析

Cloning, identification, and characterization of the rpoS-like sigma factor rpoX from Vibrio alginolyticus.

作者信息

Zhao Jing-jing, Chen Chang, Zhang Lv-ping, Hu Chao-qun

机构信息

Key Laboratory of Marine Bio-resources Sustainable Utilization (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.

出版信息

J Biomed Biotechnol. 2009;2009:126986. doi: 10.1155/2009/126986. Epub 2009 Dec 29.

DOI:10.1155/2009/126986
PMID:20069110
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2804039/
Abstract

Vibrio alginolyticus ZJ-51 displays phase variation between opaque/rugose colonies (Op) and translucent/smooth colonies (Tr). These colony variants show great differences in biofilm formation and motility. In this study, a gene encoding for an rpoS-like sigma factor, rpoX, has been cloned and characterized. The absence of rpoX did not affect colony switching rate but did decrease biofilm formation in both the Op and the Tr variants. When challenged with hydrogen peroxide, the DeltarpoX in the Op background showed a slightly higher survival rate compared with the wild type, whereas survival was decreased in the Tr background. Deletion of rpoX in the Tr background resulted in a higher ability to resist ethanol challenges and to survive hyperosmolarity challenges, and in the Op background the opposite phenotype was observed. This indicates that the rpoX gene is involved in biofilm formation and stress response but the effects are controlled by colony phase variation in V. alginolyticus.

摘要

溶藻弧菌ZJ-51在不透明/粗糙菌落（Op）和半透明/光滑菌落（Tr）之间表现出相变。这些菌落变体在生物膜形成和运动性方面存在很大差异。在本研究中，已克隆并鉴定了一个编码类rpoS σ因子rpoX的基因。rpoX的缺失不影响菌落转换率，但确实降低了Op和Tr变体中的生物膜形成。用过氧化氢处理时，Op背景中的ΔrpoX与野生型相比显示出略高的存活率，而在Tr背景中存活率降低。在Tr背景中删除rpoX导致抵抗乙醇挑战和在高渗挑战中存活的能力增强，而在Op背景中观察到相反的表型。这表明rpoX基因参与生物膜形成和应激反应，但这些影响受溶藻弧菌菌落相变的控制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/2804039/935a55779267/JBB2009-126986.006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/2804039/f4564a0ebcf5/JBB2009-126986.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/2804039/bd939ea573e1/JBB2009-126986.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/2804039/1879937c77ad/JBB2009-126986.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/2804039/64737bd24409/JBB2009-126986.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/2804039/d8f7a43541ad/JBB2009-126986.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/2804039/935a55779267/JBB2009-126986.006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/2804039/f4564a0ebcf5/JBB2009-126986.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/2804039/bd939ea573e1/JBB2009-126986.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/2804039/1879937c77ad/JBB2009-126986.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/2804039/64737bd24409/JBB2009-126986.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/2804039/d8f7a43541ad/JBB2009-126986.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/2804039/935a55779267/JBB2009-126986.006a.jpg

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