Orthopeutics, LP, Georgetown, TX 78626, USA.
J Mater Sci Mater Med. 2010 Apr;21(4):1175-81. doi: 10.1007/s10856-010-3986-8. Epub 2010 Jan 13.
We have characterized the relative efficacies of a number of protein crosslinking agents that have the potential for use in the crosslinking of proteinaceous matrices both in vitro and in vivo. The crosslinkers tested were; L: -threose (LT), Genipin (GP), Methylglyoxal (MG), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), proanthrocyanidin (PA) and glutaraldehyde (GA). The relative effectiveness of the crosslinkers with regard to their saturating concentrations was: GA > PA > EDC > MG = GP >> LT. Most of the crosslinkers displayed a pH dependence and were more effective at more alkaline pH. At optimal pH and saturating conditions, the relative reaction rates of the crosslinkers were: PA = GA > EDC > GP > MG >> LT.
我们已经对许多具有在体外和体内交联蛋白质基质潜力的蛋白质交联剂的相对效力进行了表征。测试的交联剂为：L：-苏糖醇（LT）、京尼平（GP）、甲基乙二醛（MG）、1-乙基-3-（3-二甲基氨基丙基）碳二亚胺盐酸盐（EDC）、原花青素（PA）和戊二醛（GA）。交联剂在达到饱和浓度时的相对效力为：GA > PA > EDC > MG = GP >> LT。大多数交联剂表现出 pH 依赖性，在更碱性的 pH 值下更有效。在最佳 pH 值和饱和条件下，交联剂的相对反应速率为：PA = GA > EDC > GP > MG >> LT。
