使用二氨基芪作为衍生试剂，通过毛细管电泳-质谱联用技术测定糖尿病患者血清中的乙二醛和甲基乙二醛。
Determination of glyoxal and methylglyoxal in the serum of diabetic patients by MEKC using stilbenediamine as derivatizing reagent.
作者信息
Mirza Muhammad A, Kandhro Abdul J, Memon Saima Q, Khuhawar Muhammad Y, Arain Rafee
机构信息
Dr. M. A. Kazi Institute of Chemistry, University of Sindh, Jamshoro, Pakistan.
出版信息
Electrophoresis. 2007 Nov;28(21):3940-7. doi: 10.1002/elps.200700129.
An analytical method has been developed for the separation of glyoxal (Go), methylglyoxal (MGo), and dimethylglyoxal (DMGo) by MEKC using stilbenediamine (SD) as derivatizing reagent, separation time 6.5 min, SDS as micellar medium at pH 8, and sodium tetraborate (0.1 M) as buffer. Uncoated fused-silica capillary, effective length 50 cm x 75 microm id; applied voltage 20 kV and photodiode array detection, were used. Calibration was linear within 0.02-150 microg/mL with detection limits 3.5-5.8 ng/mL. Go and MGo, observed for diabetic and healthy volunteers, were within 0.098-0.193 microg/mL Go and 0.106-0.245 microg/mL MGo with RSD 1.6-3.5 and 1.7-3.4%, respectively, in diabetics against 0.016-0.046 microg/mL Go and 0.021-0.066 microg/mL MGo with RSDs 1.5-3.5 and 1.4-3.6%, respectively, in healthy volunteers. Go and MGo in diabetics were also measured by standard addition and DMGo as an internal standard. Additives do not contribute significantly to Go and MGo matrix.
已开发出一种分析方法，用于在胶束电动色谱（MEKC）中，以二苯乙烯二胺（SD）作为衍生试剂分离乙二醛（Go）、甲基乙二醛（MGo）和二甲基乙二醛（DMGo），分离时间为6.5分钟，以十二烷基硫酸钠（SDS）作为pH 8时的胶束介质，四硼酸钠（0.1 M）作为缓冲液。使用未涂层熔融石英毛细管，有效长度50 cm×内径75μm；施加电压20 kV，并采用光电二极管阵列检测。在0.02 - 150μg/mL范围内校准呈线性，检测限为3.5 - 5.8 ng/mL。在糖尿病患者和健康志愿者中观察到，Go和MGo的含量分别为0.098 - 0.193μg/mL Go和0.106 - 0.245μg/mL MGo，相对标准偏差（RSD）分别为1.6 - 3.5%和1.7 - 3.4%；而在健康志愿者中，Go和MGo的含量分别为0.016 - 0.046μg/mL Go和0.021 - 0.066μg/mL MGo，RSD分别为1.5 - 3.5%和1.4 - 3.6%。糖尿病患者中的Go和MGo也通过标准加入法并以DMGo作为内标进行测量。添加剂对Go和MGo基质的贡献不显著。
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