Faculty of Biotechnology & Food Engineering, Technion-Israel Institute of Technology, Haifa 3200003, Israel.
Int J Mol Sci. 2021 Oct 27;22(21):11624. doi: 10.3390/ijms222111624.
Porcine extracellular matrix (pECM)-derived hydrogels were introduced, in recent years, aiming to benefit the pECM's microstructure and bioactivity, while controlling the biomaterial's physical and mechanical properties. The use of pECM from different tissues, however, offers tissue-specific features that can better serve different applications. In this study, pECM hydrogels derived from cardiac, artery, pancreas, and adipose tissues were compared in terms of composition, structure, and mechanical properties. While major similarities were demonstrated between all the pECM hydrogels, their distinctive attributes were also identified, and their substantial effects on cell-ECM interactions were revealed. Furthermore, through comprehensive protein and gene expression analyses, we show, for the first time, that each pECM hydrogel supports the spontaneous differentiation of induced pluripotent stem cells towards the resident cells of its origin tissue. These findings imply that the origin of ECM should be carefully considered when designing a biomedical platform, to achieve a maximal bioactive impact.
近年来，人们引入了猪细胞外基质（pECM）衍生水凝胶，旨在受益于 pECM 的微观结构和生物活性，同时控制生物材料的物理和机械性能。然而，使用来自不同组织的 pECM 提供了具有组织特异性的特征，可以更好地服务于不同的应用。在这项研究中，比较了源自心脏、动脉、胰腺和脂肪组织的 pECM 水凝胶在组成、结构和机械性能方面的差异。虽然所有 pECM 水凝胶之间表现出主要相似性，但也确定了它们的独特属性，并揭示了它们对细胞-ECM 相互作用的实质性影响。此外，通过全面的蛋白质和基因表达分析，我们首次表明，每种 pECM 水凝胶都支持诱导多能干细胞向其起源组织的固有细胞自发分化。这些发现表明，在设计生物医学平台时，应仔细考虑 ECM 的来源，以实现最大的生物活性影响。
