Department of General Biochemistry, University of Lodz, Lodz, Poland.
Platelets. 2009 Nov;20(7):487-92. doi: 10.3109/09537100903165182.
The effects of garcinol and guttiferone K, two polyisoprenylated benzophenones occurring a food plant called Garcinia cambogia, on oxidative/nitrative protein damage (determined by parameters such as levels of protein carbonyl groups and nitrotyrosine residues) in human blood platelets and plasma after treatment with peroxynitrite (ONOO(-)) were studied in vitro. We also investigated the effects of garcinol and guttiferone K on lipid peroxidation in blood platelets and plasma induced by ONOO(-) (100 microM). Exposure of blood platelets or plasma to peroxynitrite (100 microM) resulted in an increased level of carbonyl groups and nitrotyrosine residues in proteins, and an increase of lipid peroxidation measured by the level of thiobarbituric acid reactive species (TBARS). In the presence of garcinol and guttiferone K (0.1-25 microg/ml), a distinct reduction in the formation of carbonyl groups in plasma and platelet proteins together with the decrease of TBARS caused by 100 microM peroxynitrite, was observed. However, garcinol and guttiferone K did not inhibit plasma and platelet protein nitration induced by peroxynitrite. Polyisoprenylated benzophenones present in human diet such as garcinol or guttiferone K in vitro have protective effects against lipid and protein oxidation and may have some promising effects in vivo because they are good antioxidants in the tested models in vitro. Garcinol and guttiferone K can be also useful as protecting factors against diseases associated with oxidative stress.
两种多异戊二烯基二苯甲酮（分别存在于一种名为藤黄的食物植物中），即藤黄酚和根皮素 K，研究了其在体外对羟自由基和次氯酸（ONOO(-)）处理后人血小板和血浆中氧化/硝化蛋白质损伤（通过蛋白质羰基水平和硝基酪氨酸残基等参数确定）的影响。我们还研究了藤黄酚和根皮素 K 对 ONOO(-)（100 microM）诱导的血小板和血浆中脂质过氧化的影响。将血小板或血浆暴露于过氧亚硝酸盐（100 microM）会导致蛋白质中的羰基水平和硝基酪氨酸残基增加，并且通过硫代巴比妥酸反应性物质（TBARS）水平测量的脂质过氧化增加。在藤黄酚和根皮素 K（0.1-25 μg/ml）存在的情况下，明显减少了 100 microM 过氧亚硝酸盐引起的血浆和血小板蛋白中羰基的形成，以及 TBARS 的减少。然而，藤黄酚和根皮素 K 并没有抑制过氧亚硝酸盐诱导的血浆和血小板蛋白硝化。人类饮食中存在的多异戊二烯基二苯甲酮，如藤黄酚或根皮素 K，在体外具有抗脂质和蛋白质氧化的保护作用，并且因为它们在体外测试模型中是良好的抗氧化剂，所以可能具有一些有前途的体内效果。藤黄酚和根皮素 K 也可以作为保护因素，防止与氧化应激相关的疾病。
